Gene description for Rab8a
Gene name RAB8A, member RAS oncogene family
Gene symbol Rab8a
Other names/aliases Mel
Rab8
Species Rattus norvegicus
 Database cross references - Rab8a
ExoCarta ExoCarta_117103
Vesiclepedia VP_117103
Entrez Gene 117103
UniProt P35280  
 Rab8a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rab8a
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GTP binding GO:0005525 ISS
    GDP binding GO:0019003 IEA
    GDP binding GO:0019003 ISO
    GDP binding GO:0019003 ISS
    protein kinase binding GO:0019901 IPI
    myosin V binding GO:0031489 IEA
    myosin V binding GO:0031489 ISO
    protein tyrosine kinase binding GO:1990782 IPI
Biological Process
    exocytosis GO:0006887 IBA
    vesicle docking involved in exocytosis GO:0006904 IEA
    vesicle docking involved in exocytosis GO:0006904 ISO
    autophagy GO:0006914 IEA
    Golgi organization GO:0007030 IEA
    Golgi organization GO:0007030 ISO
    Golgi organization GO:0007030 ISS
    axonogenesis GO:0007409 IMP
    regulation of autophagy GO:0010506 IEA
    regulation of autophagy GO:0010506 ISO
    regulation of autophagy GO:0010506 ISS
    protein transport GO:0015031 IEA
    cell projection organization GO:0030030 IEA
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 ISO
    cellular response to insulin stimulus GO:0032869 IMP
    regulation of protein localization GO:0032880 IMP
    glucose homeostasis GO:0042593 IC
    positive regulation of D-glucose import GO:0046326 IC
    regulation of long-term neuronal synaptic plasticity GO:0048169 IDA
    regulation of long-term neuronal synaptic plasticity GO:0048169 IMP
    Golgi vesicle fusion to target membrane GO:0048210 IEA
    Golgi vesicle fusion to target membrane GO:0048210 ISO
    regulation of protein transport GO:0051223 IDA
    regulation of protein transport GO:0051223 IMP
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 IEA
    cilium assembly GO:0060271 ISO
    cilium assembly GO:0060271 ISS
    protein localization to cilium GO:0061512 IEA
    protein localization to cilium GO:0061512 ISO
    protein localization to cilium GO:0061512 ISS
    protein localization to plasma membrane GO:0072659 IDA
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IDA
    neurotransmitter receptor transport, endosome to postsynaptic membrane GO:0098887 IMP
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 IBA
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 IDA
    neurotransmitter receptor transport to postsynaptic membrane GO:0098969 IMP
    vesicle-mediated transport in synapse GO:0099003 IDA
    vesicle-mediated transport in synapse GO:0099003 IMP
Subcellular Localization
    lysosome GO:0005764 IEA
    endosome GO:0005768 IBA
    Golgi apparatus GO:0005794 IDA
    Golgi apparatus GO:0005794 IEA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cilium GO:0005929 IEA
    cilium GO:0005929 ISO
    cilium GO:0005929 ISS
    synaptic vesicle GO:0008021 IBA
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    trans-Golgi network transport vesicle GO:0030140 IBA
    trans-Golgi network transport vesicle GO:0030140 IEA
    trans-Golgi network transport vesicle GO:0030140 ISO
    dendrite GO:0030425 IDA
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    cytoplasmic vesicle membrane GO:0030659 TAS
    phagocytic vesicle membrane GO:0030670 IEA
    cytoplasmic vesicle GO:0031410 IDA
    ciliary basal body GO:0036064 IEA
    ciliary basal body GO:0036064 ISO
    ciliary basal body GO:0036064 ISS
    cell projection GO:0042995 IEA
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 IDA
    dendritic spine GO:0043197 IBA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    phagocytic vesicle GO:0045335 IEA
    phagocytic vesicle GO:0045335 ISO
    phagocytic vesicle GO:0045335 ISS
    recycling endosome GO:0055037 ISO
    recycling endosome membrane GO:0055038 IEA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
    ciliary membrane GO:0060170 IEA
    ciliary membrane GO:0060170 ISO
    exocytic vesicle GO:0070382 ISO
    ciliary base GO:0097546 IEA
    ciliary base GO:0097546 ISO
    non-motile cilium GO:0097730 IEA
    non-motile cilium GO:0097730 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Rab8a in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
8
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
9
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rab8a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OPTN 10133
Affinity Capture-Western Homo sapiens
2 Ocrl  
Two-hybrid Rattus norvegicus
View the network image/svg+xml



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