Gene description for Arfrp1
Gene name ADP-ribosylation factor related protein 1
Gene symbol Arfrp1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Arfrp1
ExoCarta ExoCarta_117051
Vesiclepedia VP_117051
Entrez Gene 117051
UniProt Q63055  
 Arfrp1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Arfrp1
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
Biological Process
    intracellular protein transport GO:0006886 IBA
    gastrulation GO:0007369 IEA
    gastrulation GO:0007369 ISO
    protein localization to Golgi apparatus GO:0034067 IBA
    protein localization to Golgi apparatus GO:0034067 IEA
    protein localization to Golgi apparatus GO:0034067 ISO
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    Golgi to plasma membrane protein transport GO:0043001 IBA
    Golgi to plasma membrane protein transport GO:0043001 IEA
    Golgi to plasma membrane protein transport GO:0043001 ISO
Subcellular Localization
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    cytosol GO:0005829 IEA
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
 Experiment description of studies that identified Arfrp1 in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Arfrp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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