Gene description for Rpl6
Gene name ribosomal protein L6
Gene symbol Rpl6
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rpl6
ExoCarta ExoCarta_117042
Vesiclepedia VP_117042
Entrez Gene 117042
UniProt P21533  
 Rpl6 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Rpl6
Molecular Function
    tRNA binding GO:0000049 IDA
    RNA binding GO:0003723 IBA
    mRNA binding GO:0003729 IDA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IEA
    structural constituent of ribosome GO:0003735 ISO
    5.8S rRNA binding GO:1990932 IDA
Biological Process
    cytoplasmic translation GO:0002181 IBA
    cytoplasmic translation GO:0002181 ISO
    cytoplasmic translation GO:0002181 ISS
    translation GO:0006412 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    rough endoplasmic reticulum GO:0005791 IEA
    ribosome GO:0005840 IEA
    postsynaptic density GO:0014069 ISO
    large ribosomal subunit GO:0015934 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    A band GO:0031672 IDA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    synapse GO:0045202 ISO
    cytoplasmic side of rough endoplasmic reticulum membrane GO:0098556 ISO
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified Rpl6 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rpl6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sumo3  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here