Gene description for Mapk1
Gene name mitogen activated protein kinase 1
Gene symbol Mapk1
Other names/aliases Erk2
Species Rattus norvegicus
 Database cross references - Mapk1
ExoCarta ExoCarta_116590
Vesiclepedia VP_116590
Entrez Gene 116590
UniProt P63086  
 Mapk1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Mapk1
Molecular Function
    phosphotyrosine residue binding GO:0001784 IEA
    phosphotyrosine residue binding GO:0001784 ISO
    double-stranded DNA binding GO:0003690 IDA
    protein kinase activity GO:0004672 ISO
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IC
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 ISO
    JUN kinase activity GO:0004705 IEA
    MAP kinase activity GO:0004707 IDA
    MAP kinase activity GO:0004707 IEA
    MAP kinase activity GO:0004707 IMP
    MAP kinase activity GO:0004707 ISO
    MAP kinase activity GO:0004707 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    ATP binding GO:0005524 IEA
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 IEA
    RNA polymerase II CTD heptapeptide repeat kinase activity GO:0008353 ISO
    kinase activity GO:0016301 ISO
    kinase activity GO:0016301 TAS
    protein kinase binding GO:0019901 IPI
    phosphatase binding GO:0019902 IEA
    phosphatase binding GO:0019902 ISO
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IPI
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein serine kinase activity GO:0106310 IEA
Biological Process
    MAPK cascade GO:0000165 IMP
    MAPK cascade GO:0000165 ISO
    MAPK cascade GO:0000165 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 IMP
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 TAS
    apoptotic process GO:0006915 IEA
    DNA damage response GO:0006974 IEA
    DNA damage response GO:0006974 ISO
    cell surface receptor signaling pathway GO:0007166 IBA
    JNK cascade GO:0007254 IEA
    heart development GO:0007507 ISO
    positive regulation of cell population proliferation GO:0008284 IEP
    insulin receptor signaling pathway GO:0008286 IEA
    insulin receptor signaling pathway GO:0008286 ISO
    response to toxic substance GO:0009636 IDA
    animal organ morphogenesis GO:0009887 ISO
    positive regulation of macrophage chemotaxis GO:0010759 IEA
    positive regulation of macrophage chemotaxis GO:0010759 ISO
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 ISO
    neural crest cell development GO:0014032 ISO
    Schwann cell development GO:0014044 IEA
    Schwann cell development GO:0014044 ISO
    diadenosine tetraphosphate biosynthetic process GO:0015966 IMP
    peptidyl-serine phosphorylation GO:0018105 IDA
    peptidyl-serine phosphorylation GO:0018105 IMP
    peptidyl-serine phosphorylation GO:0018105 ISO
    peptidyl-serine phosphorylation GO:0018105 ISO
    peptidyl-threonine phosphorylation GO:0018107 ISO
    peptidyl-threonine phosphorylation GO:0018107 ISS
    sensory perception of pain GO:0019233 IMP
    cytosine metabolic process GO:0019858 IEA
    cytosine metabolic process GO:0019858 ISO
    regulation of ossification GO:0030278 IEA
    regulation of ossification GO:0030278 ISO
    positive regulation of cell migration GO:0030335 IEP
    androgen receptor signaling pathway GO:0030521 IEA
    androgen receptor signaling pathway GO:0030521 ISO
    regulation of cellular pH GO:0030641 IEA
    regulation of cellular pH GO:0030641 ISO
    thyroid gland development GO:0030878 IEA
    thyroid gland development GO:0030878 ISO
    regulation of protein stability GO:0031647 ISS
    lipopolysaccharide-mediated signaling pathway GO:0031663 IEA
    lipopolysaccharide-mediated signaling pathway GO:0031663 ISO
    positive regulation of telomere maintenance GO:0032206 IEA
    positive regulation of telomere maintenance GO:0032206 ISO
    response to estradiol GO:0032355 IEP
    response to lipopolysaccharide GO:0032496 ISO
    regulation of stress-activated MAPK cascade GO:0032872 IEA
    regulation of stress-activated MAPK cascade GO:0032872 TAS
    response to testosterone GO:0033574 IEP
    mammary gland epithelial cell proliferation GO:0033598 IEA
    mammary gland epithelial cell proliferation GO:0033598 ISO
    cellular response to amino acid starvation GO:0034198 IEA
    cellular response to amino acid starvation GO:0034198 ISO
    response to nicotine GO:0035094 IEA
    response to nicotine GO:0035094 IGI
    response to nicotine GO:0035094 ISO
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    intracellular signal transduction GO:0035556 TAS
    cellular response to platelet-derived growth factor stimulus GO:0036120 IEP
    ERBB signaling pathway GO:0038127 ISO
    ERBB2-ERBB3 signaling pathway GO:0038133 IEA
    ERBB2-ERBB3 signaling pathway GO:0038133 ISO
    response to cocaine GO:0042220 IEP
    positive regulation of protein import into nucleus GO:0042307 IMP
    outer ear morphogenesis GO:0042473 IEA
    outer ear morphogenesis GO:0042473 ISO
    response to hydrogen peroxide GO:0042542 IEP
    myelination GO:0042552 IEA
    myelination GO:0042552 ISO
    response to exogenous dsRNA GO:0043330 IEA
    response to exogenous dsRNA GO:0043330 ISO
    steroid hormone receptor signaling pathway GO:0043401 ISO
    response to estrogen GO:0043627 IDA
    estrous cycle GO:0044849 IEP
    positive regulation of cholesterol biosynthetic process GO:0045542 IEA
    positive regulation of cholesterol biosynthetic process GO:0045542 ISO
    negative regulation of cell differentiation GO:0045596 IEA
    negative regulation of cell differentiation GO:0045596 ISO
    positive regulation of translation GO:0045727 IMP
    positive regulation of DNA-templated transcription GO:0045893 IMP
    decidualization GO:0046697 IDA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    insulin-like growth factor receptor signaling pathway GO:0048009 ISO
    thymus development GO:0048538 IEA
    thymus development GO:0048538 ISO
    progesterone receptor signaling pathway GO:0050847 IEA
    progesterone receptor signaling pathway GO:0050847 ISO
    T cell receptor signaling pathway GO:0050852 IEA
    T cell receptor signaling pathway GO:0050852 ISO
    B cell receptor signaling pathway GO:0050853 IEA
    B cell receptor signaling pathway GO:0050853 ISO
    stress-activated MAPK cascade GO:0051403 IEA
    stress-activated MAPK cascade GO:0051403 ISO
    regulation of cytoskeleton organization GO:0051493 IEA
    regulation of cytoskeleton organization GO:0051493 TAS
    Bergmann glial cell differentiation GO:0060020 IEA
    Bergmann glial cell differentiation GO:0060020 ISO
    positive regulation of cardiac muscle cell proliferation GO:0060045 IMP
    long-term synaptic potentiation GO:0060291 IEA
    long-term synaptic potentiation GO:0060291 ISO
    face development GO:0060324 IEA
    face development GO:0060324 ISO
    lung morphogenesis GO:0060425 IEA
    lung morphogenesis GO:0060425 ISO
    trachea formation GO:0060440 IEA
    trachea formation GO:0060440 ISO
    labyrinthine layer blood vessel development GO:0060716 IEA
    labyrinthine layer blood vessel development GO:0060716 ISO
    cardiac neural crest cell development involved in heart development GO:0061308 IEA
    cardiac neural crest cell development involved in heart development GO:0061308 ISO
    cellular response to methionine GO:0061431 IEP
    ERK1 and ERK2 cascade GO:0070371 IEA
    ERK1 and ERK2 cascade GO:0070371 IMP
    ERK1 and ERK2 cascade GO:0070371 ISO
    ERK1 and ERK2 cascade GO:0070371 ISO
    response to epidermal growth factor GO:0070849 IEA
    response to epidermal growth factor GO:0070849 ISO
    response to epidermal growth factor GO:0070849 ISS
    cellular response to cAMP GO:0071320 IEP
    cellular response to tumor necrosis factor GO:0071356 IEA
    cellular response to tumor necrosis factor GO:0071356 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 IEP
    cellular response to prostaglandin E stimulus GO:0071380 IEP
    caveolin-mediated endocytosis GO:0072584 IEA
    caveolin-mediated endocytosis GO:0072584 TAS
    regulation of Golgi inheritance GO:0090170 IEA
    regulation of Golgi inheritance GO:0090170 TAS
    cellular response to toxic substance GO:0097237 IDA
    response to alcohol GO:0097305 IEP
    positive regulation of macrophage proliferation GO:0120041 IEA
    positive regulation of macrophage proliferation GO:0120041 ISO
    cellular response to insulin-like growth factor stimulus GO:1990314 IEP
    regulation of early endosome to late endosome transport GO:2000641 IEA
    regulation of early endosome to late endosome transport GO:2000641 TAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 TAS
    early endosome GO:0005769 IEA
    early endosome GO:0005769 TAS
    late endosome GO:0005770 IEA
    late endosome GO:0005770 TAS
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 TAS
    centrosome GO:0005813 IEA
    spindle GO:0005819 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    plasma membrane GO:0005886 IDA
    caveola GO:0005901 IDA
    caveola GO:0005901 IEA
    caveola GO:0005901 TAS
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 TAS
    postsynaptic density GO:0014069 IDA
    axon GO:0030424 IDA
    pseudopodium GO:0031143 IEA
    pseudopodium GO:0031143 ISO
    dendrite cytoplasm GO:0032839 IDA
    protein-containing complex GO:0032991 IDA
    perikaryon GO:0043204 IDA
    mitotic spindle GO:0072686 ISS
 Experiment description of studies that identified Mapk1 in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Mapk1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ptgs2  
Affinity Capture-Western Rattus norvegicus
2 Scnn1b  
Biochemical Activity Rattus norvegicus
3 Mapk3 50689
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
4 Scnn1g  
Biochemical Activity Rattus norvegicus
5 Prkca  
Affinity Capture-Western Rattus norvegicus
6 PARP1 142
Biochemical Activity Homo sapiens
7 ARHGAP9 64333
Affinity Capture-MS Homo sapiens
8 Mbp  
Biochemical Activity Rattus norvegicus
9 Cnn3  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
10 Cnn1  
Reconstituted Complex Rattus norvegicus
11 HIF1A 3091
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which Mapk1 is involved
PathwayEvidenceSource
Activation of the AP-1 family of transcription factors IEA Reactome
Apoptosis IEA Reactome
Apoptotic factor-mediated response IEA Reactome
Axon guidance IEA Reactome
Ca-dependent events IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Cellular Senescence IEA Reactome
Cytochrome c-mediated apoptotic response IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Developmental Biology IEA Reactome
Downregulation of SMAD2/3:SMAD4 transcriptional activity IEA Reactome
ERK/MAPK targets IEA Reactome
ERKs are inactivated IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-dependent nuclear events downstream of ESR-membrane signaling IEA Reactome
Estrogen-stimulated signaling through PRKCZ IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
Formation of apoptosome IEA Reactome
Frs2-mediated activation IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (q) signalling events IEA Reactome
G-protein mediated events IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
Golgi Cisternae Pericentriolar Stack Reorganization IEA Reactome
GPCR downstream signalling IEA Reactome
Growth hormone receptor signaling IEA Reactome
Hemostasis IEA Reactome
IFNG signaling activates MAPKs IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
Insulin receptor signalling cascade IEA Reactome
Interferon gamma signaling IEA Reactome
Interferon Signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
Intrinsic Pathway for Apoptosis IEA Reactome
L1CAM interactions IEA Reactome
M Phase IEA Reactome
MAP kinase activation IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK targets/ Nuclear events mediated by MAP kinases IEA Reactome
MAPK1 (ERK2) activation IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MITF-M-regulated melanocyte development IEA Reactome
Mitotic Prophase IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
Negative feedback regulation of MAPK pathway IEA Reactome
Negative regulation of FGFR1 signaling IEA Reactome
Negative regulation of FGFR2 signaling IEA Reactome
Negative regulation of FGFR3 signaling IEA Reactome
Negative regulation of FGFR4 signaling IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation IEA Reactome
Nuclear Events (kinase and transcription factor activation) IEA Reactome
Oncogene Induced Senescence IEA Reactome
Opioid Signalling IEA Reactome
Oxidative Stress Induced Senescence IEA Reactome
phospho-PLA2 pathway IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
PLC beta mediated events IEA Reactome
Programmed Cell Death IEA Reactome
Prolonged ERK activation events IEA Reactome
RAF-independent MAPK1/3 activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Recycling pathway of L1 IEA Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
Regulation of the apoptosome activity IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate NADPH Oxidases IEA Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
RNA Polymerase II Transcription IEA Reactome
Senescence-Associated Secretory Phenotype (SASP) IEA Reactome
Signal attenuation IEA Reactome
Signal Transduction IEA Reactome
Signal transduction by L1 IEA Reactome
Signaling by Activin IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Insulin receptor IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by NODAL IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by TGF-beta Receptor Complex IEA Reactome
Signaling by TGFB family members IEA Reactome
Signalling to ERKs IEA Reactome
SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription IEA Reactome
Spry regulation of FGF signaling IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll-like Receptor Cascades IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
Transcriptional activity of SMAD2/SMAD3:SMAD4 heterotrimer IEA Reactome
Transcriptional and post-translational regulation of MITF-M expression and activity IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome





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