Gene description for Ak1
Gene name adenylate kinase 1
Gene symbol Ak1
Other names/aliases Ak-1
B430205N08Rik
Species Mus musculus
 Database cross references - Ak1
ExoCarta ExoCarta_11636
Vesiclepedia VP_11636
Entrez Gene 11636
UniProt Q9R0Y5  
 Ak1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ak1
Molecular Function
    adenylate kinase activity GO:0004017 IBA
    adenylate kinase activity GO:0004017 IDA
    adenylate kinase activity GO:0004017 ISO
    nucleoside diphosphate kinase activity GO:0004550 ISO
    nucleoside diphosphate kinase activity GO:0004550 ISS
    ATP binding GO:0005524 IEA
    phosphotransferase activity, phosphate group as acceptor GO:0016776 ISO
    (deoxy)adenylate kinase activity GO:0047506 IEA
Biological Process
    ADP biosynthetic process GO:0006172 IEA
    ADP biosynthetic process GO:0006172 ISO
    nucleoside triphosphate biosynthetic process GO:0009142 IEA
    positive regulation of D-glucose transmembrane transport GO:0010828 ISO
    AMP metabolic process GO:0046033 IEA
    AMP metabolic process GO:0046033 ISO
    ATP metabolic process GO:0046034 IEA
    inosine biosynthetic process GO:0046103 ISO
    nucleoside monophosphate phosphorylation GO:0046940 IEA
Subcellular Localization
    outer dense fiber GO:0001520 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    sarcomere GO:0030017 ISO
    sperm flagellum GO:0036126 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Ak1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ak1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Reconstituted Complex Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 MAPT  
Reconstituted Complex Homo sapiens
5 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
6 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ak1 is involved
PathwayEvidenceSource
Interconversion of nucleotide di- and triphosphates IEA Reactome
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome





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