Gene description for GBP4
Gene name guanylate binding protein 4
Gene symbol GBP4
Other names/aliases Mpa2
Species Homo sapiens
 Database cross references - GBP4
ExoCarta ExoCarta_115361
Vesiclepedia VP_115361
Entrez Gene 115361
HGNC 20480
MIM 612466
UniProt Q96PP9  
 GBP4 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for GBP4
Molecular Function
    GTPase activity GO:0003924 IBA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
    membrane scission GTPase motor activity GO:1990606 IEA
Biological Process
    defense response to protozoan GO:0042832 IBA
    defense response to Gram-positive bacterium GO:0050830 IBA
    cellular response to type II interferon GO:0071346 IBA
    cellular response to type II interferon GO:0071346 IEP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    nucleus GO:0005634 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    cytoplasmic vesicle GO:0031410 IBA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified GBP4 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GBP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which GBP4 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Immune System TAS Reactome
Interferon gamma signaling TAS Reactome
Interferon Signaling TAS Reactome





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