Gene description for Mapre1
Gene name microtubule-associated protein, RP/EB family, member 1
Gene symbol Mapre1
Other names/aliases Eb1
Species Rattus norvegicus
 Database cross references - Mapre1
ExoCarta ExoCarta_114764
Vesiclepedia VP_114764
Entrez Gene 114764
UniProt Q66HR2  
 Mapre1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Mapre1
Molecular Function
    microtubule binding GO:0008017 IEA
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    microtubule plus-end binding GO:0051010 IBA
    microtubule plus-end binding GO:0051010 IDA
    microtubule plus-end binding GO:0051010 IEA
    microtubule plus-end binding GO:0051010 ISO
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IEA
    establishment of mitotic spindle orientation GO:0000132 ISO
    establishment of mitotic spindle orientation GO:0000132 ISS
    microtubule bundle formation GO:0001578 ISO
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    regulation of microtubule polymerization or depolymerization GO:0031110 IBA
    negative regulation of microtubule polymerization GO:0031115 IEA
    negative regulation of microtubule polymerization GO:0031115 ISO
    positive regulation of microtubule polymerization GO:0031116 IEA
    positive regulation of microtubule polymerization GO:0031116 ISO
    protein localization to microtubule GO:0035372 IBA
    protein localization to microtubule GO:0035372 ISO
    protein localization to microtubule GO:0035372 ISO
    protein localization to microtubule GO:0035372 ISS
    microtubule polymerization GO:0046785 ISO
    spindle assembly GO:0051225 IBA
    cell division GO:0051301 IEA
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 IEA
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 ISO
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 ISS
    protein localization to centrosome GO:0071539 ISO
    protein localization to mitotic spindle GO:1902480 IEA
    protein localization to mitotic spindle GO:1902480 ISO
    protein localization to astral microtubule GO:1902888 IEA
    protein localization to astral microtubule GO:1902888 ISO
    protein localization to astral microtubule GO:1902888 ISS
    non-motile cilium assembly GO:1905515 ISO
Subcellular Localization
    spindle pole GO:0000922 IEA
    Golgi apparatus GO:0005794 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    microtubule organizing center GO:0005815 IBA
    spindle GO:0005819 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISS
    microtubule GO:0005874 ISS
    cytoplasmic microtubule GO:0005881 IBA
    focal adhesion GO:0005925 IEA
    focal adhesion GO:0005925 ISO
    microtubule cytoskeleton GO:0015630 ISO
    cortical microtubule cytoskeleton GO:0030981 IEA
    cortical microtubule cytoskeleton GO:0030981 ISO
    cell projection membrane GO:0031253 ISO
    microtubule plus-end GO:0035371 IBA
    microtubule plus-end GO:0035371 ISO
    ciliary basal body GO:0036064 ISO
    cell projection GO:0042995 ISO
    spindle midzone GO:0051233 IBA
    mitotic spindle pole GO:0097431 IEA
    mitotic spindle pole GO:0097431 ISO
    mitotic spindle pole GO:0097431 ISS
    mitotic spindle astral microtubule end GO:1905721 IEA
    mitotic spindle astral microtubule end GO:1905721 ISO
    mitotic spindle microtubule GO:1990498 IEA
    mitotic spindle microtubule GO:1990498 ISO
 Experiment description of studies that identified Mapre1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Mapre1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Katna1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Mapre1 is involved
PathwayEvidenceSource
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
G2/M Transition IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint IEA Reactome





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