Gene description for Ddx39b
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
Gene symbol Ddx39b
Other names/aliases Bat1
Bat1a
p47
Species Rattus norvegicus
 Database cross references - Ddx39b
ExoCarta ExoCarta_114612
Vesiclepedia VP_114612
Entrez Gene 114612
UniProt Q63413  
 Ddx39b identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ddx39b
Molecular Function
    nucleic acid binding GO:0003676 IEA
    DNA clamp loader activity GO:0003689 IEA
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 IEA
    RNA helicase activity GO:0003724 ISO
    mRNA binding GO:0003729 IBA
    ATP binding GO:0005524 IEA
    ATP-dependent activity, acting on RNA GO:0008186 ISO
    hydrolase activity GO:0016787 IEA
    ATP hydrolysis activity GO:0016887 ISO
    U6 snRNA binding GO:0017070 IEA
    U6 snRNA binding GO:0017070 ISO
    U4 snRNA binding GO:0030621 IEA
    U4 snRNA binding GO:0030621 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    ATP-dependent protein binding GO:0043008 IEA
    ATP-dependent protein binding GO:0043008 ISO
    protein-containing complex binding GO:0044877 IDA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    spliceosomal complex assembly GO:0000245 IEA
    spliceosomal complex assembly GO:0000245 ISO
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 ISO
    liver development GO:0001889 IEP
    chromatin remodeling GO:0006338 IEA
    mRNA processing GO:0006397 IEA
    mRNA export from nucleus GO:0006406 IBA
    mRNA export from nucleus GO:0006406 IEA
    mRNA export from nucleus GO:0006406 ISO
    RNA splicing GO:0008380 IEA
    RNA splicing GO:0008380 ISO
    RNA secondary structure unwinding GO:0010501 IEA
    RNA secondary structure unwinding GO:0010501 ISO
    positive regulation of translation GO:0045727 IMP
    mRNA transport GO:0051028 IEA
    positive regulation of cell growth involved in cardiac muscle cell development GO:0061051 IMP
    chromatin looping GO:0140588 IEA
    positive regulation of vascular associated smooth muscle cell proliferation GO:1904707 IMP
    positive regulation of DNA biosynthetic process GO:2000573 IMP
Subcellular Localization
    transcription export complex GO:0000346 IEA
    transcription export complex GO:0000346 ISO
    nucleus GO:0005634 ISO
    spliceosomal complex GO:0005681 IEA
    spliceosomal complex GO:0005681 ISO
    U4 snRNP GO:0005687 IEA
    U4 snRNP GO:0005687 ISO
    U6 snRNP GO:0005688 IEA
    U6 snRNP GO:0005688 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    nuclear matrix GO:0016363 IDA
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified Ddx39b in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ddx39b
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sumo3  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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