Gene description for Sec13
Gene name SEC13 homolog (S. cerevisiae)
Gene symbol Sec13
Other names/aliases 1110003H02Rik
Sec13l1
Sec13r
Species Mus musculus
 Database cross references - Sec13
ExoCarta ExoCarta_110379
Vesiclepedia VP_110379
Entrez Gene 110379
UniProt Q9D1M0  
 Sec13 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sec13
Molecular Function
    structural molecule activity GO:0005198 IBA
    structural molecule activity GO:0005198 IEA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    protein import into nucleus GO:0006606 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISO
    nucleocytoplasmic transport GO:0006913 NAS
    cellular response to nutrient levels GO:0031669 ISO
    cellular response to nutrient levels GO:0031669 ISS
    positive regulation of TOR signaling GO:0032008 IBA
    protein exit from endoplasmic reticulum GO:0032527 IBA
    protein exit from endoplasmic reticulum GO:0032527 IMP
    mRNA transport GO:0051028 IEA
    protein localization to plasma membrane GO:0072659 ISS
    COPII-coated vesicle cargo loading GO:0090110 IEA
    COPII-coated vesicle cargo loading GO:0090110 ISO
    COPII-coated vesicle budding GO:0090114 IBA
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of TORC1 signaling GO:1904263 ISS
Subcellular Localization
    kinetochore GO:0000776 IEA
    kinetochore GO:0000776 ISO
    nuclear envelope GO:0005635 ISO
    nuclear pore GO:0005643 ISO
    nuclear pore GO:0005643 NAS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IEA
    COPII vesicle coat GO:0030127 IBA
    COPII vesicle coat GO:0030127 IEA
    COPII vesicle coat GO:0030127 ISO
    nuclear pore outer ring GO:0031080 IBA
    nuclear pore outer ring GO:0031080 ISO
    nuclear pore outer ring GO:0031080 ISS
    protein-containing complex GO:0032991 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    GATOR2 complex GO:0061700 ISO
    GATOR2 complex GO:0061700 ISS
 Experiment description of studies that identified Sec13 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sec13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cenpi  
Co-fractionation Mus musculus
2 Clasp1  
Co-fractionation Mus musculus
3 Stam2  
Co-fractionation Mus musculus
4 Spag5  
Co-fractionation Mus musculus
5 Sec24a  
Co-fractionation Mus musculus
6 Tpr 108989
Co-fractionation Mus musculus
7 Zfp207  
Co-fractionation Mus musculus
8 Zw10  
Co-fractionation Mus musculus
9 Foxp3  
Affinity Capture-MS Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 Ckap5 75786
Co-fractionation Mus musculus
12 Aaas  
Co-fractionation Mus musculus
13 Nupl2  
Co-fractionation Mus musculus
14 Cenpe  
Co-fractionation Mus musculus
15 Sec31a  
Co-fractionation Mus musculus
16 Trappc12  
Co-fractionation Mus musculus
17 Rangap1  
Co-fractionation Mus musculus
18 Nup107  
Co-fractionation Mus musculus
19 Meaf6  
Co-fractionation Mus musculus
20 Nanog  
Affinity Capture-MS Mus musculus
21 Atg5  
Affinity Capture-RNA Mus musculus
22 Dynll1 56455
Co-fractionation Mus musculus
23 Sec23b  
Co-fractionation Mus musculus
24 Sgol1  
Co-fractionation Mus musculus
25 Sec23a  
Co-fractionation Mus musculus
26 Atg16l1  
Affinity Capture-MS Mus musculus
27 Mad1l1  
Co-fractionation Mus musculus
28 Seh1l 72124
Co-fractionation Mus musculus
29 Cenph  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Sec13 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Amino acids regulate mTORC1 IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Antigen Presentation: Folding, assembly and peptide loading of class I MHC IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular response to heat stress IEA Reactome
Cellular response to starvation IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Class I MHC mediated antigen processing & presentation IEA Reactome
COPII-mediated vesicle transport IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
Gene expression (Transcription) IEA Reactome
Gene Silencing by RNA IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Immune System IEA Reactome
M Phase IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome
Metabolism of non-coding RNA IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of RNA IEA Reactome
MHC class II antigen presentation IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Prophase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Nuclear Envelope (NE) Reassembly IEA Reactome
Nuclear Envelope Breakdown IEA Reactome
Nuclear Pore Complex (NPC) Disassembly IEA Reactome
Post-translational protein modification IEA Reactome
Postmitotic nuclear pore complex (NPC) reformation IEA Reactome
Processing of Capped Intron-Containing Pre-mRNA IEA Reactome
Regulation of Glucokinase by Glucokinase Regulatory Protein IEA Reactome
Regulation of HSF1-mediated heat shock response IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
snRNP Assembly IEA Reactome
SUMO E3 ligases SUMOylate target proteins IEA Reactome
SUMOylation IEA Reactome
SUMOylation of chromatin organization proteins IEA Reactome
SUMOylation of DNA damage response and repair proteins IEA Reactome
SUMOylation of RNA binding proteins IEA Reactome
SUMOylation of SUMOylation proteins IEA Reactome
SUMOylation of ubiquitinylation proteins IEA Reactome
Transcriptional regulation by small RNAs IEA Reactome
Transport of Mature mRNA derived from an Intron-Containing Transcript IEA Reactome
Transport of Mature mRNA Derived from an Intronless Transcript IEA Reactome
Transport of Mature mRNAs Derived from Intronless Transcripts IEA Reactome
Transport of Mature Transcript to Cytoplasm IEA Reactome
Transport of the SLBP Dependant Mature mRNA IEA Reactome
Transport of the SLBP independent Mature mRNA IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





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