Gene description for B4gat1
Gene name beta-1,4-glucuronyltransferase 1
Gene symbol B4gat1
Other names/aliases 1500032M01Rik
B3gnt1
B3gnt6
BETA3GNT1
iGAT
iGNT
Species Mus musculus
 Database cross references - B4gat1
ExoCarta ExoCarta_108902
Vesiclepedia VP_108902
Entrez Gene 108902
UniProt Q8BWP8  
 B4gat1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for B4gat1
Molecular Function
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity GO:0008532 ISS
    glucuronosyltransferase activity GO:0015020 IBA
    glucuronosyltransferase activity GO:0015020 ISO
    glucuronosyltransferase activity GO:0015020 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    protein glycosylation GO:0006486 IMP
    axon guidance GO:0007411 IMP
    poly-N-acetyllactosamine biosynthetic process GO:0030311 ISS
    protein O-linked mannosylation GO:0035269 IBA
    protein O-linked mannosylation GO:0035269 ISO
    protein O-linked mannosylation GO:0035269 ISS
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
 Experiment description of studies that identified B4gat1 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for B4gat1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo6  
Co-fractionation Mus musculus
View the network image/svg+xml



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