Gene description for Napg
Gene name N-ethylmaleimide sensitive fusion protein attachment protein gamma
Gene symbol Napg
Other names/aliases 2400003O04Rik
SNARE
Species Mus musculus
 Database cross references - Napg
ExoCarta ExoCarta_108123
Vesiclepedia VP_108123
Entrez Gene 108123
UniProt Q9CWZ7  
 Napg identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Napg
Molecular Function
    soluble NSF attachment protein activity GO:0005483 IBA
    syntaxin binding GO:0019905 IBA
Biological Process
    intracellular protein transport GO:0006886 IBA
    intracellular protein transport GO:0006886 IEA
    vesicle-mediated transport GO:0016192 IEA
Subcellular Localization
    mitochondrion GO:0005739 ISO
    Golgi apparatus GO:0005794 IEA
    membrane GO:0016020 IEA
    SNARE complex GO:0031201 IBA
    myelin sheath GO:0043209 HDA
    synapse GO:0045202 ISO
 Experiment description of studies that identified Napg in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Napg
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bcl2  
Affinity Capture-MS Mus musculus
2 Rab11fip5  
Affinity Capture-Western Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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