Gene description for Otub1
Gene name OTU domain, ubiquitin aldehyde binding 1
Gene symbol Otub1
Other names/aliases AI850305
Species Mus musculus
 Database cross references - Otub1
ExoCarta ExoCarta_107260
Vesiclepedia VP_107260
Entrez Gene 107260
UniProt Q7TQI3  
 Otub1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Otub1
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    cysteine-type deubiquitinase activity GO:0004843 ISS
    deNEDDylase activity GO:0019784 ISO
    deNEDDylase activity GO:0019784 ISS
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    ubiquitin binding GO:0043130 IBA
    ubiquitin binding GO:0043130 ISO
    ubiquitin binding GO:0043130 ISS
    ubiquitin-protein transferase inhibitor activity GO:0055105 ISO
    ubiquitin-protein transferase inhibitor activity GO:0055105 ISS
    deubiquitinase activity GO:0101005 ISO
Biological Process
    adaptive immune response GO:0002250 IEA
    DNA repair GO:0006281 IEA
    proteolysis GO:0006508 IEA
    DNA damage response GO:0006974 IBA
    DNA damage response GO:0006974 ISO
    DNA damage response GO:0006974 ISS
    protein deubiquitination GO:0016579 ISO
    protein deubiquitination GO:0016579 ISO
    protein deubiquitination GO:0016579 ISS
    protein K48-linked deubiquitination GO:0071108 ISO
    protein K48-linked deubiquitination GO:0071108 ISS
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of TORC1 signaling GO:1904263 ISS
    negative regulation of double-strand break repair GO:2000780 ISO
    negative regulation of double-strand break repair GO:2000780 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified Otub1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Otub1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ubc  
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
2 Smad3  
Affinity Capture-MS Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Wdr82  
Co-fractionation Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Ndn  
Two-hybrid Mus musculus
7 Smad2  
Affinity Capture-MS Mus musculus
8 MAPT  
Reconstituted Complex Homo sapiens
9 Mapt  
Affinity Capture-MS Mus musculus
10 MTNR1A  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
11 Tia1  
Affinity Capture-MS Mus musculus
12 Fancd2  
Affinity Capture-MS Mus musculus
13 Usp8  
Co-fractionation Mus musculus
View the network image/svg+xml



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