Gene description for Smpdl3b
Gene name sphingomyelin phosphodiesterase, acid-like 3B
Gene symbol Smpdl3b
Other names/aliases 1110054A24Rik
AU045240
Asml3b
Species Mus musculus
 Database cross references - Smpdl3b
ExoCarta ExoCarta_100340
Vesiclepedia VP_100340
Entrez Gene 100340
UniProt P58242  
 Smpdl3b identified in sEVs derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Smpdl3b
Molecular Function
    sphingomyelin phosphodiesterase activity GO:0004767 IEA
    protein binding GO:0005515 IPI
    phosphoric diester hydrolase activity GO:0008081 IBA
    phosphoric diester hydrolase activity GO:0008081 IDA
    phosphoric diester hydrolase activity GO:0008081 IMP
    phosphoric diester hydrolase activity GO:0008081 ISO
    zinc ion binding GO:0008270 IDA
    hydrolase activity, acting on glycosyl bonds GO:0016798 IEA
Biological Process
    sphingomyelin catabolic process GO:0006685 IEA
    inflammatory response GO:0006954 IEA
    negative regulation of toll-like receptor signaling pathway GO:0034122 IMP
    innate immune response GO:0045087 IEA
    negative regulation of innate immune response GO:0045824 TAS
    membrane lipid catabolic process GO:0046466 IMP
    negative regulation of inflammatory response GO:0050728 IMP
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IEA
    plasma membrane GO:0005886 IDA
    side of membrane GO:0098552 IEA
 Experiment description of studies that identified Smpdl3b in sEVs
1
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Smpdl3b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Smpdl3b is involved
No pathways found





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