ExoCarta: Gene summary

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Gene description for Adh5
Gene name	alcohol dehydrogenase 5 (class III), chi polypeptide
Gene symbol	Adh5
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Adh5
ExoCarta	ExoCarta_100145871
Vesiclepedia	VP_100145871
Entrez Gene	100145871
UniProt	P12711
Adh5 identified in sEVs derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Adh5

Molecular Function
	alcohol dehydrogenase (NAD+) activity	GO:0004022	IBA
	alcohol dehydrogenase (NAD+) activity	GO:0004022	IDA
	alcohol dehydrogenase (NAD+) activity	GO:0004022	IEA
	alcohol dehydrogenase (NAD+) activity	GO:0004022	ISO
	fatty acid binding	GO:0005504	ISO
	zinc ion binding	GO:0008270	IBA
	zinc ion binding	GO:0008270	IEA
	zinc ion binding	GO:0008270	ISO
	oxidoreductase activity	GO:0016491	IEA
	formaldehyde dehydrogenase (NAD+) activity	GO:0018467	ISO
	identical protein binding	GO:0042802	ISO
	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity	GO:0051903	IBA
	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity	GO:0051903	IEA
	S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity	GO:0051903	ISO
	S-nitrosoglutathione reductase (NADH) activity	GO:0080007	IEA
	S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity	GO:0106321	IEA
	S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity	GO:0106322	IEA
Biological Process
	retinoid metabolic process	GO:0001523	ISO
	respiratory system process	GO:0003016	ISO
	ethanol catabolic process	GO:0006068	IDA
	fatty acid omega-oxidation	GO:0010430	ISO
	fatty acid omega-oxidation	GO:0010430	ISS
	peptidyl-cysteine S-nitrosylation	GO:0018119	ISO
	response to lipopolysaccharide	GO:0032496	ISO
	positive regulation of blood pressure	GO:0045777	ISO
	formaldehyde catabolic process	GO:0046294	IBA
	formaldehyde catabolic process	GO:0046294	IEA
	formaldehyde catabolic process	GO:0046294	ISO
	response to nitrosative stress	GO:0051409	ISO
	response to redox state	GO:0051775	ISO
Subcellular Localization
	cytoplasm	GO:0005737	IEA
	mitochondrion	GO:0005739	ISO
	cytosol	GO:0005829	IBA

Experiment description of studies that identified Adh5 in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D7 Rep 1

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.13-1.25 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting