Gene description for PEX16
Gene name peroxisomal biogenesis factor 16
Gene symbol PEX16
Other names/aliases PBD8A
PBD8B
Species Homo sapiens
 Database cross references - PEX16
ExoCarta ExoCarta_9409
Vesiclepedia VP_9409
Entrez Gene 9409
HGNC 8857
MIM 603360
UniProt Q9Y5Y5  
 PEX16 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PEX16
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    protein targeting to peroxisome GO:0006625 IMP
    peroxisome organization GO:0007031 IBA
    peroxisome organization GO:0007031 IMP
    peroxisome membrane biogenesis GO:0016557 IMP
    protein import into peroxisome matrix GO:0016558 IMP
    protein to membrane docking GO:0022615 IDA
    ER-dependent peroxisome organization GO:0032581 IDA
    protein import into peroxisome membrane GO:0045046 IMP
    ER-dependent peroxisome localization GO:0106101 IDA
Subcellular Localization
    peroxisome GO:0005777 IDA
    peroxisomal membrane GO:0005778 HDA
    peroxisomal membrane GO:0005778 IBA
    peroxisomal membrane GO:0005778 IDA
    peroxisomal membrane GO:0005778 IMP
    peroxisomal membrane GO:0005778 TAS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified PEX16 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PEX16
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAGEA8  
Affinity Capture-MS Homo sapiens
2 SLC39A12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
4 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 SLC10A6  
Two-hybrid Homo sapiens
6 SLC2A12 154091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TTYH1  
Affinity Capture-MS Homo sapiens
8 LEPROTL1 23484
Two-hybrid Homo sapiens
9 FFAR2  
Two-hybrid Homo sapiens
10 C3AR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LDLRAD1  
Affinity Capture-MS Homo sapiens
12 PEX19 5824
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 DEXI  
Two-hybrid Homo sapiens
14 PTH2R  
Affinity Capture-MS Homo sapiens
15 FCER1G 2207
Two-hybrid Homo sapiens
16 ERGIC3 51614
Two-hybrid Homo sapiens
17 BSCL2  
Affinity Capture-MS Homo sapiens
18 GPR12  
Affinity Capture-MS Homo sapiens
19 ADCY8  
Affinity Capture-MS Homo sapiens
20 CAND1 55832
Affinity Capture-MS Homo sapiens
21 CD79A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 AQP3  
Affinity Capture-MS Homo sapiens
23 OPRL1 4987
Affinity Capture-MS Homo sapiens
24 CREB3L1  
Two-hybrid Homo sapiens
25 KIAA1467 57613
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 REEP4  
Two-hybrid Homo sapiens
27 GPR152  
Two-hybrid Homo sapiens
28 SLC18A2  
Affinity Capture-MS Homo sapiens
29 PGRMC2 10424
Two-hybrid Homo sapiens
30 MICB  
Affinity Capture-MS Homo sapiens
31 MMGT1 93380
Two-hybrid Homo sapiens
32 ATP2A1 487
Affinity Capture-MS Homo sapiens
33 TMX2 51075
Two-hybrid Homo sapiens
34 SLC10A1  
Two-hybrid Homo sapiens
35 PEX14 5195
Co-purification Homo sapiens
36 PDZK1IP1 10158
Two-hybrid Homo sapiens
37 KCNJ6  
Two-hybrid Homo sapiens
38 GLMP 112770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 EBAG9 9166
Two-hybrid Homo sapiens
40 BDKRB2  
Two-hybrid Homo sapiens
41 PEX3 8504
Proximity Label-MS Homo sapiens
42 SCN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 AKAP1 8165
Proximity Label-MS Homo sapiens
44 ZDHHC15 158866
Two-hybrid Homo sapiens
45 HIATL1 84641
Two-hybrid Homo sapiens
46 ACBD5 91452
Proximity Label-MS Homo sapiens
47 CLEC10A  
Two-hybrid Homo sapiens
View the network image/svg+xml



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