Gene description for APH1B
Gene name APH1B gamma secretase subunit
Gene symbol APH1B
Other names/aliases APH-1B
PRO1328
PSFL
TAAV688
Species Homo sapiens
 Database cross references - APH1B
ExoCarta ExoCarta_83464
Vesiclepedia VP_83464
Entrez Gene 83464
HGNC 24080
MIM 607630
UniProt Q8WW43  
 APH1B identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for APH1B
Molecular Function
    protein binding GO:0005515 IPI
    protein-macromolecule adaptor activity GO:0030674 IBA
    protein-macromolecule adaptor activity GO:0030674 IMP
    endopeptidase activator activity GO:0061133 IGI
    endopeptidase activator activity GO:0061133 IMP
Biological Process
    Notch signaling pathway GO:0007219 IBA
    Notch receptor processing GO:0007220 IBA
    Notch receptor processing GO:0007220 IDA
    Notch receptor processing GO:0007220 NAS
    positive regulation of endopeptidase activity GO:0010950 IGI
    positive regulation of endopeptidase activity GO:0010950 IMP
    protein processing GO:0016485 IBA
    protein processing GO:0016485 IDA
    protein processing GO:0016485 IDA
    protein processing GO:0016485 IGI
    membrane protein intracellular domain proteolysis GO:0031293 ISS
    amyloid-beta formation GO:0034205 IDA
    amyloid-beta formation GO:0034205 IGI
    amyloid precursor protein catabolic process GO:0042987 IDA
    amyloid precursor protein catabolic process GO:0042987 IGI
    amyloid precursor protein catabolic process GO:0042987 ISS
Subcellular Localization
    Golgi membrane GO:0000139 NAS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 NAS
    plasma membrane GO:0005886 ISS
    plasma membrane GO:0005886 TAS
    endosome membrane GO:0010008 TAS
    membrane GO:0016020 IDA
    transport vesicle GO:0030133 IDA
    gamma-secretase complex GO:0070765 IBA
    gamma-secretase complex GO:0070765 IDA
    gamma-secretase complex GO:0070765 IGI
    gamma-secretase complex GO:0070765 ISS
 Experiment description of studies that identified APH1B in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for APH1B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSEN2 5664
Affinity Capture-Western Homo sapiens
2 TUSC5  
Two-hybrid Homo sapiens
3 TM4SF4  
Two-hybrid Homo sapiens
4 VSTM1  
Two-hybrid Homo sapiens
5 PSEN1 5663
Affinity Capture-Western Homo sapiens
6 NCSTN 23385
Affinity Capture-Western Homo sapiens
7 FXYD6-FXYD2  
Two-hybrid Homo sapiens
8 PSENEN  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which APH1B is involved
PathwayEvidenceSource
Activated NOTCH1 Transmits Signal to the Nucleus IEA Reactome
Amyloid fiber formation TAS Reactome
Axon guidance TAS Reactome
Cell death signalling via NRAGE, NRIF and NADE TAS Reactome
Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants TAS Reactome
Constitutive Signaling by NOTCH1 PEST Domain Mutants TAS Reactome
Death Receptor Signaling TAS Reactome
Developmental Biology TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
EPH-ephrin mediated repulsion of cells TAS Reactome
EPH-Ephrin signaling TAS Reactome
Metabolism of proteins TAS Reactome
Nervous system development TAS Reactome
Noncanonical activation of NOTCH3 TAS Reactome
NOTCH2 Activation and Transmission of Signal to the Nucleus IEA Reactome
NOTCH3 Activation and Transmission of Signal to the Nucleus TAS Reactome
NOTCH4 Activation and Transmission of Signal to the Nucleus IEA Reactome
NRIF signals cell death from the nucleus TAS Reactome
Nuclear signaling by ERBB4 TAS Reactome
p75 NTR receptor-mediated signalling TAS Reactome
Regulated proteolysis of p75NTR TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by NOTCH IEA Reactome
Signaling by NOTCH TAS Reactome
Signaling by NOTCH1 IEA Reactome
Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH1 in Cancer TAS Reactome
Signaling by NOTCH1 PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH2 IEA Reactome
Signaling by NOTCH3 TAS Reactome
Signaling by NOTCH4 IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by TGFB family members TAS Reactome
Signaling by TGFBR3 TAS Reactome
TGFBR3 PTM regulation TAS Reactome





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