Gene description for TSHR
Gene name thyroid stimulating hormone receptor
Gene symbol TSHR
Other names/aliases CHNG1
LGR3
hTSHR-I
Species Homo sapiens
 Database cross references - TSHR
ExoCarta ExoCarta_7253
Vesiclepedia VP_7253
Entrez Gene 7253
HGNC 12373
MIM 603372
UniProt P16473  
 TSHR identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for TSHR
Molecular Function
    thyroid-stimulating hormone receptor activity GO:0004996 IBA
    thyroid-stimulating hormone receptor activity GO:0004996 IMP
    protein binding GO:0005515 IPI
    G protein-coupled peptide receptor activity GO:0008528 IBA
    signaling receptor activity GO:0038023 IMP
    protein-containing complex binding GO:0044877 IDA
Biological Process
    cell surface receptor signaling pathway GO:0007166 IMP
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger GO:0007187 TAS
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IMP
    cell-cell signaling GO:0007267 TAS
    positive regulation of cell population proliferation GO:0008284 TAS
    hormone-mediated signaling pathway GO:0009755 IBA
    thyroid-stimulating hormone signaling pathway GO:0038194 IMP
    positive regulation of cold-induced thermogenesis GO:0120162 ISS
    cellular response to glycoprotein GO:1904588 IMP
    cellular response to thyrotropin-releasing hormone GO:1905229 IMP
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 ISS
    basolateral plasma membrane GO:0016323 IDA
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified TSHR in sEVs
1
Experiment ID 211
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TSHR
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TMEM119 338773
Two-hybrid Homo sapiens
2 ART5  
Affinity Capture-MS Homo sapiens
3 LAMB1 3912
Affinity Capture-MS Homo sapiens
4 FBXO7 25793
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TUBA1A 7846
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 SALL2  
Affinity Capture-MS Homo sapiens
7 HSPA5 3309
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
8 NGLY1 55768
Affinity Capture-MS Homo sapiens
9 TUBB 203068
Affinity Capture-MS Homo sapiens
10 TIMP1 7076
Affinity Capture-MS Homo sapiens
11 FGFR1 2260
Affinity Capture-MS Homo sapiens
12 PCSK6 5046
Affinity Capture-MS Homo sapiens
13 ADGRG1 9289
Affinity Capture-MS Homo sapiens
14 TCP11L1 55346
Affinity Capture-MS Homo sapiens
15 CANX 821
Affinity Capture-Western Homo sapiens
16 MAN2B2 23324
Affinity Capture-MS Homo sapiens
17 LAMA3 3909
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 JPH3 57338
Two-hybrid Homo sapiens
19 OS9 10956
Affinity Capture-MS Homo sapiens
20 MOCOS  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 SPSB3  
Affinity Capture-MS Homo sapiens
22 POLD3  
Affinity Capture-MS Homo sapiens
23 TUBB4A 10382
Affinity Capture-MS Homo sapiens
24 ADRB2  
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
25 NEU1 4758
Affinity Capture-MS Homo sapiens
26 CDC42EP1 11135
Affinity Capture-MS Homo sapiens
27 TUBB8 347688
Affinity Capture-MS Homo sapiens
28 NOMO1 23420
Affinity Capture-MS Homo sapiens
29 LMAN2L 81562
Affinity Capture-MS Homo sapiens
30 PVR 5817
Affinity Capture-MS Homo sapiens
31 FAM132B  
Affinity Capture-MS Homo sapiens
32 BNIP1 662
Two-hybrid Homo sapiens
33 SAMD1  
Affinity Capture-MS Homo sapiens
34 PTDSS1 9791
Two-hybrid Homo sapiens
35 HNRNPA1 3178
Two-hybrid Homo sapiens
36 RHOT2 89941
Affinity Capture-MS Homo sapiens
37 MOG  
Two-hybrid Homo sapiens
38 ESRP2 80004
Affinity Capture-MS Homo sapiens
39 ASPHD1  
Two-hybrid Homo sapiens
40 FN1 2335
Reconstituted Complex Homo sapiens
41 PLXNB2 23654
Affinity Capture-MS Homo sapiens
42 POTEF 728378
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 GOLGA2 2801
Affinity Capture-MS Homo sapiens
44 MLF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TUBA4A 7277
Affinity Capture-MS Homo sapiens
46 LAMC1 3915
Affinity Capture-MS Homo sapiens
47 MID1IP1  
Two-hybrid Homo sapiens
48 CD81 975
Two-hybrid Homo sapiens
49 ITGA7 3679
Affinity Capture-MS Homo sapiens
50 CSPG4 1464
Affinity Capture-MS Homo sapiens
51 CHMP4B 128866
Affinity Capture-MS Homo sapiens
52 TMEM132A 54972
Affinity Capture-MS Homo sapiens
53 PC 5091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 SCO2  
Affinity Capture-MS Homo sapiens
55 IQGAP2 10788
Affinity Capture-MS Homo sapiens
56 ITPA 3704
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 JAG2  
Affinity Capture-MS Homo sapiens
58 POMK  
Affinity Capture-MS Homo sapiens
59 LAMB2 3913
Affinity Capture-MS Homo sapiens
60 CHRNA5  
Affinity Capture-MS Homo sapiens
61 TRMT44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 CRELD2 79174
Affinity Capture-MS Homo sapiens
63 BORA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FLNB 2317
Protein-peptide Homo sapiens
65 SCRIB 23513
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
66 ACTB 60
Two-hybrid Homo sapiens
67 CAMK2D 817
Affinity Capture-MS Homo sapiens
68 STAT3 6774
Affinity Capture-Western Homo sapiens
69 STIL  
Affinity Capture-MS Homo sapiens
70 ADORA1  
Two-hybrid Homo sapiens
71 RUNDC3A  
Two-hybrid Homo sapiens
72 JAK2 3717
Affinity Capture-Western Homo sapiens
73 CBWD1 55871
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 COMMD4 54939
Affinity Capture-MS Homo sapiens
75 CRADD  
Two-hybrid Homo sapiens
76 OAF 220323
Affinity Capture-MS Homo sapiens
77 TMCO3 55002
Two-hybrid Homo sapiens
78 JAK1 3716
Affinity Capture-Western Homo sapiens
79 SYT1 6857
Two-hybrid Homo sapiens
80 ERAL1  
Affinity Capture-MS Homo sapiens
81 PTK7 5754
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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