Gene description for Cotl1
Gene name coactosin-like 1 (Dictyostelium)
Gene symbol Cotl1
Other names/aliases 1810074P22Rik
2010004C08Rik
Clp
Species Mus musculus
 Database cross references - Cotl1
ExoCarta ExoCarta_72042
Vesiclepedia VP_72042
Entrez Gene 72042
UniProt Q9CQI6  
 Cotl1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cotl1
Molecular Function
    actin binding GO:0003779 IDA
    actin binding GO:0003779 ISO
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    enzyme binding GO:0019899 ISO
    actin filament binding GO:0051015 IBA
Biological Process
    regulation of actin filament polymerization GO:0030833 IBA
    defense response to fungus GO:0050832 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 IEA
    actin filament GO:0005884 IBA
    site of polarized growth GO:0030427 IBA
    cortical actin cytoskeleton GO:0030864 IBA
 Experiment description of studies that identified Cotl1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cotl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Arrb2  
Co-fractionation Mus musculus
3 Atxn1  
Affinity Capture-MS Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 ALOX5  
Reconstituted Complex Homo sapiens
6 Actg1 11465
Co-fractionation Mus musculus
Two-hybrid Mus musculus
7 Actb 11461
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Cotl1 is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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