Gene description for Sptan1
Gene name spectrin, alpha, non-erythrocytic 1
Gene symbol Sptan1
Other names/aliases A2a
IPF
Spna2
Species Rattus norvegicus
 Database cross references - Sptan1
ExoCarta ExoCarta_64159
Vesiclepedia VP_64159
Entrez Gene 64159
UniProt P16086  
 Sptan1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Sptan1
Molecular Function
    actin binding GO:0003779 IEA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    spectrin binding GO:0030507 IDA
    spectrin binding GO:0030507 ISO
    protein-containing complex binding GO:0044877 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    actin cytoskeleton organization GO:0030036 IBA
    actin filament capping GO:0051693 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    fascia adherens GO:0005916 ISO
    spectrin GO:0008091 TAS
    microtubule cytoskeleton GO:0015630 IEA
    microtubule cytoskeleton GO:0015630 ISO
    membrane GO:0016020 ISO
    lateral plasma membrane GO:0016328 ISO
    Z disc GO:0030018 ISO
    cell junction GO:0030054 IBA
    cortical cytoskeleton GO:0030863 ISO
    cortical actin cytoskeleton GO:0030864 IBA
    cortical actin cytoskeleton GO:0030864 IEA
    cuticular plate GO:0032437 ISO
    protein-containing complex GO:0032991 IDA
    paranodal junction GO:0033010 ISO
    paranode region of axon GO:0033270 ISO
    cell projection GO:0042995 IBA
    intracellular membrane-bounded organelle GO:0043231 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    postsynapse GO:0098794 IDA
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Sptan1 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Sptan1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Epb41l2  
Affinity Capture-Western Rattus norvegicus
2 Epb4.1  
Affinity Capture-Western Rattus norvegicus
3 Actn1 81634
Affinity Capture-Western Rattus norvegicus
4 Shank3  
Affinity Capture-Western Rattus norvegicus
5 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
6 Grin2b  
Co-fractionation Rattus norvegicus
View the network image/svg+xml



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