Gene description for Stk4
Gene name serine/threonine kinase 4
Gene symbol Stk4
Other names/aliases AI447339
AU020804
Kas-2
Mst1
Ysk3
Species Mus musculus
 Database cross references - Stk4
ExoCarta ExoCarta_58231
Vesiclepedia VP_58231
Entrez Gene 58231
UniProt Q9JI11  
 Stk4 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Stk4
Molecular Function
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    protein kinase activity GO:0004672 ISO
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISO
    protein serine/threonine kinase activity GO:0004674 ISS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 ISO
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    cell morphogenesis GO:0000902 ISO
    branching involved in blood vessel morphogenesis GO:0001569 IGI
    neural tube formation GO:0001841 IGI
    positive regulation of protein phosphorylation GO:0001934 ISO
    endocardium development GO:0003157 IGI
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISO
    protein import into nucleus GO:0006606 IEA
    protein import into nucleus GO:0006606 ISO
    apoptotic process GO:0006915 IGI
    apoptotic process GO:0006915 ISO
    apoptotic process GO:0006915 ISS
    central nervous system development GO:0007417 IGI
    cell population proliferation GO:0008283 IGI
    negative regulation of cell population proliferation GO:0008285 IGI
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IGI
    peptidyl-serine phosphorylation GO:0018105 ISO
    keratinocyte differentiation GO:0030216 IGI
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    organ growth GO:0035265 IGI
    hippo signaling GO:0035329 IGI
    hippo signaling GO:0035329 IMP
    hippo signaling GO:0035329 ISO
    positive regulation of hippo signaling GO:0035332 ISO
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 ISO
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of apoptotic process GO:0043065 IGI
    positive regulation of apoptotic process GO:0043065 ISO
    regulation of MAPK cascade GO:0043408 IBA
    positive regulation of fat cell differentiation GO:0045600 IGI
    negative regulation of organ growth GO:0046621 IGI
    protein autophosphorylation GO:0046777 ISO
    epithelial cell proliferation GO:0050673 IGI
    negative regulation of epithelial cell proliferation GO:0050680 IGI
    protein stabilization GO:0050821 IMP
    protein stabilization GO:0050821 ISO
    protein tetramerization GO:0051262 IEA
    canonical Wnt signaling pathway GO:0060070 IGI
    primitive hemopoiesis GO:0060215 IGI
    cell differentiation involved in embryonic placenta development GO:0060706 IGI
    regulation of cell differentiation involved in embryonic placenta development GO:0060800 IGI
    negative regulation of canonical Wnt signaling pathway GO:0090090 IBA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IGI
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISO
    hepatocyte apoptotic process GO:0097284 IGI
    hepatocyte apoptotic process GO:0097284 IGI
    positive regulation of extrinsic apoptotic signaling pathway via death domain receptors GO:1902043 IGI
    positive regulation of hepatocyte apoptotic process GO:1903945 IGI
    positive regulation of substrate-dependent cell migration, cell attachment to substrate GO:1904237 IMP
    positive regulation of vascular associated smooth muscle cell apoptotic process GO:1905461 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    nuclear body GO:0016604 IEA
    nuclear body GO:0016604 ISO
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Stk4 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Stk4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Map1s  
Co-fractionation Mus musculus
4 Eed  
Affinity Capture-MS Mus musculus
5 Rabgef1  
Co-fractionation Mus musculus
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 Tma16  
Co-fractionation Mus musculus
8 Strn 268980
Co-fractionation Mus musculus
9 Fancd2  
Affinity Capture-MS Mus musculus
10 Daxx  
Affinity Capture-Western Mus musculus
11 Eif4ebp1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Stk4 is involved
PathwayEvidenceSource
Signal Transduction IEA Reactome
Signaling by Hippo IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here