Gene description for PTGIS
Gene name prostaglandin I2 (prostacyclin) synthase
Gene symbol PTGIS
Other names/aliases CYP8
CYP8A1
PGIS
PTGI
Species Homo sapiens
 Database cross references - PTGIS
ExoCarta ExoCarta_5740
Vesiclepedia VP_5740
Entrez Gene 5740
HGNC 9603
MIM 601699
UniProt Q16647  
 PTGIS identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for PTGIS
Molecular Function
    monooxygenase activity GO:0004497 IEA
    iron ion binding GO:0005506 IEA
    protein binding GO:0005515 IPI
    prostaglandin-I synthase activity GO:0008116 IBA
    prostaglandin-I synthase activity GO:0008116 IDA
    prostaglandin-I synthase activity GO:0008116 TAS
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen GO:0016705 IEA
    heme binding GO:0020037 IDA
    hydroperoxy icosatetraenoate dehydratase activity GO:0106256 IEA
Biological Process
    prostaglandin biosynthetic process GO:0001516 IBA
    prostaglandin biosynthetic process GO:0001516 IDA
    icosanoid metabolic process GO:0006690 IDA
    icosanoid metabolic process GO:0006690 TAS
    cyclooxygenase pathway GO:0019371 TAS
    negative regulation of NF-kappaB transcription factor activity GO:0032088 IDA
    NAD biosynthesis via nicotinamide riboside salvage pathway GO:0034356 TAS
    positive regulation of peroxisome proliferator activated receptor signaling pathway GO:0035360 IDA
    negative regulation of nitric oxide biosynthetic process GO:0045019 IDA
    positive regulation of angiogenesis GO:0045766 IMP
    negative regulation of inflammatory response GO:0050728 IDA
    cellular response to interleukin-1 GO:0071347 IEP
    cellular response to interleukin-6 GO:0071354 IEP
    cellular response to hypoxia GO:0071456 IDA
    apoptotic signaling pathway GO:0097190 IDA
    positive regulation of execution phase of apoptosis GO:1900119 IDA
Subcellular Localization
    extracellular space GO:0005615 ISS
    nucleus GO:0005634 IDA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 ISS
    endoplasmic reticulum membrane GO:0005789 TAS
    caveola GO:0005901 IDA
 Experiment description of studies that identified PTGIS in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PTGIS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTGS2 5743
FRET Homo sapiens
2 PTGS1 5742
FRET Homo sapiens
3 APP 351
Reconstituted Complex Homo sapiens
4 CAV1 857
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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