Gene description for PRSS2
Gene name protease, serine, 2 (trypsin 2)
Gene symbol PRSS2
Other names/aliases TRY2
TRY8
TRYP2
Species Homo sapiens
 Database cross references - PRSS2
ExoCarta ExoCarta_5645
Vesiclepedia VP_5645
Entrez Gene 5645
HGNC 9483
MIM 601564
UniProt P07478  
 PRSS2 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for PRSS2
Molecular Function
    metalloendopeptidase activity GO:0004222 TAS
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    serine-type endopeptidase activity GO:0004252 TAS
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    serine-type peptidase activity GO:0008236 TAS
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IDA
    digestion GO:0007586 IEA
    antimicrobial humoral response GO:0019730 TAS
    extracellular matrix disassembly GO:0022617 TAS
    positive regulation of cell growth GO:0030307 TAS
    collagen catabolic process GO:0030574 IDA
    collagen catabolic process GO:0030574 IMP
    collagen catabolic process GO:0030574 TAS
    positive regulation of cell adhesion GO:0045785 TAS
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IMP
    extracellular matrix GO:0031012 TAS
    azurophil granule lumen GO:0035578 TAS
 Experiment description of studies that identified PRSS2 in sEVs
1
Experiment ID 494
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PRSS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PYGO1  
Affinity Capture-MS Homo sapiens
2 HSPA5 3309
Affinity Capture-MS Homo sapiens
3 HOXA2  
Proximity Label-MS Homo sapiens
4 SLC35A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CD8B 926
Affinity Capture-MS Homo sapiens
6 TUBB3 10381
Affinity Capture-MS Homo sapiens
7 RIN3  
Affinity Capture-MS Homo sapiens
8 METRN 79006
Affinity Capture-MS Homo sapiens
9 PRSS3 5646
Affinity Capture-MS Homo sapiens
10 GLP1R  
PCA Homo sapiens
11 KIF20A 10112
Affinity Capture-MS Homo sapiens
12 USP11 8237
Affinity Capture-MS Homo sapiens
13 FUBP3 8939
Affinity Capture-MS Homo sapiens
14 TUBB1 81027
Affinity Capture-MS Homo sapiens
15 SBDS 51119
Affinity Capture-MS Homo sapiens
16 FN1 2335
Affinity Capture-MS Homo sapiens
17 SYDE1 85360
Affinity Capture-MS Homo sapiens
18 POTEF 728378
Affinity Capture-MS Homo sapiens
19 FCER1A  
Affinity Capture-MS Homo sapiens
20 BTBD1  
Affinity Capture-MS Homo sapiens
21 CSN1S1 1446
Biochemical Activity Homo sapiens
22 GDF11 10220
Affinity Capture-MS Homo sapiens
23 CIDEC  
Affinity Capture-MS Homo sapiens
24 PRSS1 5644
Affinity Capture-MS Homo sapiens
25 SUMF1 285362
Affinity Capture-MS Homo sapiens
26 GSTT1 2952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TMEM252  
Affinity Capture-MS Homo sapiens
28 BMP1 649
Affinity Capture-MS Homo sapiens
29 ARSK  
Affinity Capture-MS Homo sapiens
30 DKK2  
Affinity Capture-MS Homo sapiens
31 ITGA4 3676
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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