Experiment ID |
12 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
MHCI
|
Enriched markers |
✔
CANX|cytochrome c
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
15478216
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of melanoma-derived exosomes by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. |
Authors |
"Mears R, Craven RA, Hanrahan S, Totty N, Upton C, Young SL, Patel P, Selby PJ, Banks RE" |
Journal name |
PROTEOMICS
|
Publication year |
2004 |
Sample |
Melanoma cells |
Sample name |
MeWo SK-MEL-28 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.20 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [4700 Proteomics Analyzer] Western blotting |