Gene description for RHOT1
Gene name ras homolog family member T1
Gene symbol RHOT1
Other names/aliases ARHT1
MIRO-1
MIRO1
Species Homo sapiens
 Database cross references - RHOT1
ExoCarta ExoCarta_55288
Vesiclepedia VP_55288
Entrez Gene 55288
HGNC 21168
MIM 613888
UniProt Q8IXI2  
 RHOT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for RHOT1
Molecular Function
    GTPase activity GO:0003924 IBA
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
Biological Process
    mitochondrion organization GO:0007005 IBA
    regulation of mitochondrion organization GO:0010821 IMP
    cellular homeostasis GO:0019725 IMP
    mitochondrion transport along microtubule GO:0047497 IBA
    mitochondrion transport along microtubule GO:0047497 IMP
    mitochondrial outer membrane permeabilization GO:0097345 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 NAS
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 IDA
    mitochondrial outer membrane GO:0005741 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified RHOT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RHOT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FTL 2512
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 MAP2K2 5605
Affinity Capture-MS Homo sapiens
4 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 SPRTN  
Affinity Capture-MS Homo sapiens
7 IPO5 3843
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TRAK1 22906
Affinity Capture-MS Homo sapiens
9 KLK5 25818
Affinity Capture-MS Homo sapiens
10 NUBP2 10101
Affinity Capture-MS Homo sapiens
11 PEX19 5824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GZMH  
Affinity Capture-MS Homo sapiens
13 TRIM66  
Affinity Capture-MS Homo sapiens
14 MTCH2 23788
Proximity Label-MS Homo sapiens
15 MGARP  
Affinity Capture-MS Homo sapiens
16 IRAK1  
Affinity Capture-MS Homo sapiens
17 ERLIN2 11160
Affinity Capture-MS Homo sapiens
18 PARK2  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
19 AMFR 267
Affinity Capture-MS Homo sapiens
20 FFAR1  
Affinity Capture-MS Homo sapiens
21 SOX2  
Affinity Capture-MS Homo sapiens
22 NXF1 10482
Affinity Capture-RNA Homo sapiens
23 APEX1 328
Affinity Capture-RNA Homo sapiens
24 PPTC7 160760
Affinity Capture-MS Homo sapiens
25 APOOL 139322
Affinity Capture-MS Homo sapiens
26 NTRK1 4914
Affinity Capture-MS Homo sapiens
27 COQ9  
Affinity Capture-MS Homo sapiens
28 PINK1  
Affinity Capture-Western Homo sapiens
29 GPR182  
Affinity Capture-MS Homo sapiens
30 TRDN 10345
Affinity Capture-MS Homo sapiens
31 PCSK9 255738
Affinity Capture-MS Homo sapiens
32 ILK 3611
Affinity Capture-MS Homo sapiens
33 DDRGK1 65992
Affinity Capture-MS Homo sapiens
34 ISLR 3671
Affinity Capture-MS Homo sapiens
35 PTPN18 26469
Affinity Capture-MS Homo sapiens
36 SP7  
Affinity Capture-MS Homo sapiens
37 RHOT2 89941
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 ATP2A3 489
Affinity Capture-MS Homo sapiens
39 AKAP1 8165
Proximity Label-MS Homo sapiens
40 ASB14  
Affinity Capture-MS Homo sapiens
41 HUWE1 10075
Affinity Capture-MS Homo sapiens
42 PIPSL 266971
Affinity Capture-MS Homo sapiens
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