Gene description for KIF5C
Gene name kinesin family member 5C
Gene symbol KIF5C
Other names/aliases CDCBM2
KINN
NKHC
NKHC-2
NKHC2
Species Homo sapiens
 Database cross references - KIF5C
ExoCarta ExoCarta_3800
Vesiclepedia VP_3800
Entrez Gene 3800
HGNC 6325
MIM 604593
UniProt O60282  
 KIF5C identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Neuroblastoma cells 25944692    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for KIF5C
Molecular Function
    microtubule motor activity GO:0003777 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    microtubule binding GO:0008017 IBA
    plus-end-directed microtubule motor activity GO:0008574 IBA
    ATP hydrolysis activity GO:0016887 IBA
    apolipoprotein receptor binding GO:0034190 IEA
Biological Process
    organelle organization GO:0006996 TAS
    axon guidance GO:0007411 IBA
    motor neuron axon guidance GO:0008045 IEA
    intracellular mRNA localization GO:0008298 IEA
    synaptic vesicle transport GO:0048489 IBA
    mRNA transport GO:0051028 ISS
    anterograde dendritic transport of messenger ribonucleoprotein complex GO:0098964 IEA
    anterograde dendritic transport of neurotransmitter receptor complex GO:0098971 IBA
    anterograde axonal protein transport GO:0099641 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    kinesin complex GO:0005871 IBA
    microtubule GO:0005874 IBA
    dendrite cytoplasm GO:0032839 IEA
    ciliary rootlet GO:0035253 IEA
    neuronal cell body GO:0043025 ISS
    axonal growth cone GO:0044295 ISS
    GABA-ergic synapse GO:0098982 IEA
    postsynaptic cytosol GO:0099524 IEA
    distal axon GO:0150034 ISS
    axon cytoplasm GO:1904115 IEA
 Experiment description of studies that identified KIF5C in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KIF5C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HVCN1 84329
Affinity Capture-MS Homo sapiens
3 TRIP6 7205
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CUL1 8454
Affinity Capture-MS Homo sapiens
5 HOOK1  
Proximity Label-MS Homo sapiens
6 KLC2 64837
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
7 ILF3 3609
Affinity Capture-RNA Homo sapiens
8 TP53 7157
Affinity Capture-MS Homo sapiens
9 SYCE1  
Affinity Capture-MS Homo sapiens
10 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 C15orf59  
Affinity Capture-MS Homo sapiens
12 KLC3  
Affinity Capture-MS Homo sapiens
13 NUP62 23636
Affinity Capture-MS Homo sapiens
14 SYNE4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 YWHAZ 7534
Affinity Capture-MS Homo sapiens
16 CSNK2A1 1457
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
17 KLC1 3831
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TCERG1 10915
Cross-Linking-MS (XL-MS) Homo sapiens
19 AZIN1 51582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HOMER1  
Affinity Capture-MS Homo sapiens
21 KLC4 89953
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
22 BRK1 55845
Affinity Capture-MS Homo sapiens
23 SNAPIN 23557
Two-hybrid Homo sapiens
24 COG6 57511
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 KIF5A 3798
Cross-Linking-MS (XL-MS) Homo sapiens
26 ANKMY2 57037
Co-fractionation Homo sapiens
27 NTRK1 4914
Affinity Capture-MS Homo sapiens
28 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 KIF5B 3799
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-Western Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
30 MEOX2  
Two-hybrid Homo sapiens
31 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
33 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MYCL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 KPTN  
Affinity Capture-MS Homo sapiens
36 CBY1  
Affinity Capture-MS Homo sapiens
37 MAGEA3  
Affinity Capture-MS Homo sapiens
38 RANBP2 5903
Affinity Capture-Western Homo sapiens
39 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KIF5C is involved
PathwayEvidenceSource
Insulin processing IEA Reactome
Metabolism of proteins IEA Reactome
Peptide hormone metabolism IEA Reactome





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