Gene description for APOF
Gene name apolipoprotein F
Gene symbol APOF
Other names/aliases Apo-F
LTIP
Species Homo sapiens
 Database cross references - APOF
ExoCarta ExoCarta_319
Vesiclepedia VP_319
Entrez Gene 319
HGNC 615
MIM 107760
UniProt Q13790  
 APOF identified in sEVs derived from the following tissue/cell type
Plasma 36624553    
Plasma 36624553    
 Gene ontology annotations for APOF
Molecular Function
    signaling receptor binding GO:0005102 TAS
    lipid transporter activity GO:0005319 TAS
    protein binding GO:0005515 IPI
    cholesterol binding GO:0015485 TAS
Biological Process
    lipid metabolic process GO:0006629 TAS
    lipid transport GO:0006869 IEA
    cholesterol metabolic process GO:0008203 IBA
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    low-density lipoprotein particle GO:0034362 IEA
    high-density lipoprotein particle GO:0034364 IEA
 Experiment description of studies that identified APOF in sEVs
1
Experiment ID 606
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 606
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 607
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 607
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36624553    
Organism Homo sapiens
Experiment description Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors "Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name Proteomics
Publication year 2023
Sample Plasma
Sample name Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods Filtration
Size exclusion chromatography
Ion exchange adsorption
Centrifugation
Centrifugal ultrafiltration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for APOF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GALNT4 8693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 GAPDHS 26330
Affinity Capture-MS Homo sapiens
3 APOA1 335
Co-purification Homo sapiens
4 APOA2 336
Co-purification Homo sapiens
View the network image/svg+xml



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