Gene description for HMGCS2
Gene name 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial)
Gene symbol HMGCS2
Other names/aliases -
Species Homo sapiens
 Database cross references - HMGCS2
ExoCarta ExoCarta_3158
Vesiclepedia VP_3158
Entrez Gene 3158
HGNC 5008
MIM 600234
UniProt P54868  
 HMGCS2 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for HMGCS2
Molecular Function
    hydroxymethylglutaryl-CoA synthase activity GO:0004421 IBA
    hydroxymethylglutaryl-CoA synthase activity GO:0004421 IMP
    hydroxymethylglutaryl-CoA synthase activity GO:0004421 ISS
    hydroxymethylglutaryl-CoA synthase activity GO:0004421 TAS
    identical protein binding GO:0042802 IDA
Biological Process
    kidney development GO:0001822 IEA
    liver development GO:0001889 IEA
    acetyl-CoA metabolic process GO:0006084 IBA
    acetyl-CoA metabolic process GO:0006084 IMP
    cholesterol biosynthetic process GO:0006695 IEA
    midgut development GO:0007494 IEA
    response to nutrient GO:0007584 IEA
    response to temperature stimulus GO:0009266 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    response to metal ion GO:0010038 IEA
    farnesyl diphosphate biosynthetic process, mevalonate pathway GO:0010142 IBA
    lung development GO:0030324 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    multicellular organismal response to stress GO:0033555 IEA
    response to testosterone GO:0033574 IEA
    response to glucagon GO:0033762 IEA
    response to triglyceride GO:0034014 IEA
    response to monosaccharide GO:0034284 IEA
    response to prostaglandin F GO:0034696 IEA
    response to starvation GO:0042594 IEA
    response to ethanol GO:0045471 IEA
    ketone body biosynthetic process GO:0046951 IMP
    ketone body biosynthetic process GO:0046951 TAS
    response to cAMP GO:0051591 IEA
    response to growth hormone GO:0060416 IEA
    adipose tissue development GO:0060612 IEA
    response to linoleic acid GO:0070543 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to amino acid stimulus GO:0071230 IEA
    cellular response to glucocorticoid stimulus GO:0071385 IEA
    cellular response to fatty acid GO:0071398 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified HMGCS2 in sEVs
1
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for HMGCS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 NFATC1 4772
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SSR3 6747
Affinity Capture-MS Homo sapiens
5 HSD17B10 3028
Two-hybrid Homo sapiens
6 YWHAQ 10971
Affinity Capture-MS Homo sapiens
7 CUL4A 8451
Affinity Capture-MS Homo sapiens
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