Experiment description of studies that identified ANXA2P2 in sEVs

1 Experiment ID 1221 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 31054213     Organism Homo sapiens Experiment description Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma Authors "Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C." Journal name Proteomics Publication year 2019 Sample Cholangiocarcinoma cells Sample name KKU-M213 Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 1222 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 31054213     Organism Homo sapiens Experiment description Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma Authors "Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C." Journal name Proteomics Publication year 2019 Sample Cholangiocarcinoma cells Sample name KKU-M213D5 Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Sucrose density gradient centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 20 MISEV standards ✔ EM Biophysical techniques ✔ HSP90|CD63|CD81|LAMP1 Enriched markers ✔ GOLGA2|cytochrome c Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 17956143     Organism Homo sapiens Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells. Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS" Journal name JPR Publication year 2007 Sample Colorectal cancer cells Sample name HT29 Isolation/purification methods Differential centrifugation Sucrose density gradient Diafiltration Flotation density 1.16 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

4 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

5 Experiment ID 236 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name MHCC97L Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

6 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

8 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

9 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

10 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for ANXA2P2

Protein Interactor ExoCarta ID Identification method PubMed Species 1 ANLN 54443 Affinity Capture-MS Homo sapiens 2 HNRNPC 3183 Cross-Linking-MS (XL-MS) Homo sapiens 3 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 4 MYCN   Affinity Capture-MS Homo sapiens 5 USP53 54532 Affinity Capture-MS Homo sapiens 6 KIF14 9928 Affinity Capture-MS Homo sapiens 7 KIF20A 10112 Affinity Capture-MS Homo sapiens 8 USP1 7398 Affinity Capture-MS Homo sapiens 9 ECT2 1894 Affinity Capture-MS Homo sapiens 10 MCM5 4174 Affinity Capture-MS Homo sapiens 11 RPA2 6118 Affinity Capture-MS Homo sapiens 12 RPA1 6117 Affinity Capture-MS Homo sapiens 13 SMURF1 57154 Affinity Capture-MS Homo sapiens 14 PARK2   Affinity Capture-MS Homo sapiens 15 FUS 2521 Affinity Capture-MS Homo sapiens 16 UFL1 23376 Affinity Capture-MS Homo sapiens 17 CHMP4C 92421 Affinity Capture-MS Homo sapiens 18 CHMP4B 128866 Affinity Capture-MS Homo sapiens 19 RPA3 6119 Affinity Capture-MS Homo sapiens 20 KIF23 9493 Affinity Capture-MS Homo sapiens 21 MCM2 4171 Affinity Capture-MS Homo sapiens 22 PINK1   Affinity Capture-MS Homo sapiens 23 PRC1 9055 Affinity Capture-MS Homo sapiens 24 C9orf72   Affinity Capture-MS Homo sapiens 25 ANXA2 302 Cross-Linking-MS (XL-MS) Homo sapiens 26 CUL2 8453 Affinity Capture-MS Homo sapiens 27 DDX3X 1654 Affinity Capture-MS Homo sapiens 28 H2AFX 3014 Affinity Capture-MS Homo sapiens View the network

Pathways in which ANXA2P2 is involved

No pathways found