Gene description for HAS1
Gene name hyaluronan synthase 1
Gene symbol HAS1
Other names/aliases HAS
Species Homo sapiens
 Database cross references - HAS1
ExoCarta ExoCarta_3036
Vesiclepedia VP_3036
Entrez Gene 3036
HGNC 4818
MIM 601463
UniProt Q92839  
 HAS1 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for HAS1
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    hyaluronan synthase activity GO:0050501 IBA
    hyaluronan synthase activity GO:0050501 ISS
    hyaluronan synthase activity GO:0050501 TAS
Biological Process
    polysaccharide biosynthetic process GO:0000271 IBA
    polysaccharide biosynthetic process GO:0000271 ISS
    glycosaminoglycan biosynthetic process GO:0006024 TAS
    cell adhesion GO:0007155 TAS
    negative regulation of fibroblast migration GO:0010764 IMP
    hyaluronan biosynthetic process GO:0030213 IBA
    hyaluronan biosynthetic process GO:0030213 IEA
    hyaluronan biosynthetic process GO:0030213 TAS
    cellular response to platelet-derived growth factor stimulus GO:0036120 IDA
    extracellular matrix assembly GO:0085029 IBA
    extracellular matrix assembly GO:0085029 ISS
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified HAS1 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HAS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 Pparg  
Affinity Capture-MS Mus musculus
3 DDX1 1653
Co-fractionation Homo sapiens
4 EWSR1 2130
Co-fractionation Homo sapiens
5 VHL  
Affinity Capture-MS Homo sapiens
6 CEMIP 57214
Affinity Capture-MS Homo sapiens
7 MCM2 4171
Affinity Capture-MS Homo sapiens
8 EIF6 3692
Affinity Capture-MS Homo sapiens
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