Gene description for Capzb
Gene name capping protein (actin filament) muscle Z-line, beta
Gene symbol Capzb
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Capzb
ExoCarta ExoCarta_298584
Vesiclepedia VP_298584
Entrez Gene 298584
UniProt Q5XI32  
 Capzb identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Capzb
Molecular Function
    actin binding GO:0003779 IEA
    actin binding GO:0003779 ISO
    actin binding GO:0003779 ISS
    protein binding GO:0005515 IPI
    actin filament binding GO:0051015 IBA
Biological Process
    cell morphogenesis GO:0000902 IBA
    cytoskeleton organization GO:0007010 ISO
    cytoskeleton organization GO:0007010 ISS
    biological_process GO:0008150 ND
    actin polymerization or depolymerization GO:0008154 IEA
    actin polymerization or depolymerization GO:0008154 ISO
    regulation of lamellipodium assembly GO:0010591 IBA
    regulation of cell morphogenesis GO:0022604 IEA
    regulation of cell morphogenesis GO:0022604 ISO
    regulation of cell morphogenesis GO:0022604 ISS
    cell projection organization GO:0030030 ISO
    lamellipodium assembly GO:0030032 IEA
    lamellipodium assembly GO:0030032 ISO
    actin cytoskeleton organization GO:0030036 IEA
    barbed-end actin filament capping GO:0051016 IBA
    barbed-end actin filament capping GO:0051016 IEA
    negative regulation of filopodium assembly GO:0051490 IBA
Subcellular Localization
    brush border GO:0005903 IEA
    brush border GO:0005903 ISO
    F-actin capping protein complex GO:0008290 IBA
    F-actin capping protein complex GO:0008290 IEA
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
    sarcomere GO:0030017 IEA
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    cortical cytoskeleton GO:0030863 IEA
    cortical cytoskeleton GO:0030863 ISO
    asymmetric synapse GO:0032279 ISO
    neuronal cell body GO:0043025 IDA
    dendritic spine GO:0043197 IDA
    WASH complex GO:0071203 IEA
    WASH complex GO:0071203 ISO
    WASH complex GO:0071203 ISS
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    Schaffer collateral - CA1 synapse GO:0098685 ISO
    hippocampal mossy fiber to CA3 synapse GO:0098686 IEA
    hippocampal mossy fiber to CA3 synapse GO:0098686 ISO
    sperm head-tail coupling apparatus GO:0120212 IEA
    sperm head-tail coupling apparatus GO:0120212 ISO
 Experiment description of studies that identified Capzb in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Capzb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dab1  
Affinity Capture-Western Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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