Gene description for Cd47
Gene name Cd47 molecule
Gene symbol Cd47
Other names/aliases Itgp
Species Rattus norvegicus
 Database cross references - Cd47
ExoCarta ExoCarta_29364
Vesiclepedia VP_29364
Entrez Gene 29364
UniProt P97829  
 Cd47 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Cd47
Molecular Function
    fibrinogen binding GO:0070051 ISO
    thrombospondin receptor activity GO:0070053 IBA
    thrombospondin receptor activity GO:0070053 IEA
    thrombospondin receptor activity GO:0070053 ISO
    protein binding involved in heterotypic cell-cell adhesion GO:0086080 IPI
    cell-cell adhesion mediator activity GO:0098632 ISO
Biological Process
    inflammatory response GO:0006954 ISO
    cell adhesion GO:0007155 IEA
    integrin-mediated signaling pathway GO:0007229 IEA
    opsonization GO:0008228 ISO
    positive regulation of cell population proliferation GO:0008284 ISO
    response to bacterium GO:0009617 ISO
    cell migration GO:0016477 IDA
    positive regulation of cell-cell adhesion GO:0022409 IBA
    positive regulation of cell-cell adhesion GO:0022409 IEA
    positive regulation of cell-cell adhesion GO:0022409 ISO
    regulation of type II interferon production GO:0032649 ISO
    regulation of interleukin-10 production GO:0032653 ISO
    regulation of interleukin-12 production GO:0032655 ISO
    regulation of interleukin-6 production GO:0032675 ISO
    regulation of tumor necrosis factor production GO:0032680 ISO
    heterotypic cell-cell adhesion GO:0034113 IEA
    regulation of nitric oxide biosynthetic process GO:0045428 ISO
    positive regulation of inflammatory response GO:0050729 IBA
    positive regulation of inflammatory response GO:0050729 IEA
    positive regulation of inflammatory response GO:0050729 ISO
    negative regulation of phagocytosis GO:0050765 IMP
    positive regulation of phagocytosis GO:0050766 IBA
    positive regulation of phagocytosis GO:0050766 IEA
    positive regulation of phagocytosis GO:0050766 IMP
    positive regulation of phagocytosis GO:0050766 ISO
    positive regulation of T cell activation GO:0050870 ISO
    positive regulation of stress fiber assembly GO:0051496 IMP
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 IMP
    positive regulation of activation-induced cell death of T cells GO:0070237 ISO
    monocyte aggregation GO:0070487 ISO
    cellular response to type II interferon GO:0071346 IMP
    cellular response to interleukin-1 GO:0071347 IMP
    cellular response to interleukin-12 GO:0071349 ISO
    ATP export GO:1904669 ISO
    regulation of synapse pruning GO:1905806 ISO
    positive regulation of monocyte extravasation GO:2000439 IMP
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    extracellular exosome GO:0070062 IBA
    extracellular exosome GO:0070062 ISO
    presynapse GO:0098793 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Cd47 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 101
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Cd47
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
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