Gene description for GJA5
Gene name gap junction protein, alpha 5, 40kDa
Gene symbol GJA5
Other names/aliases ATFB11
CX40
Species Homo sapiens
 Database cross references - GJA5
ExoCarta ExoCarta_2702
Vesiclepedia VP_2702
Entrez Gene 2702
HGNC 4279
MIM 121013
UniProt P36382  
 GJA5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GJA5
Molecular Function
    protein binding GO:0005515 IPI
    gap junction hemi-channel activity GO:0055077 IDA
    connexin binding GO:0071253 IEA
    gap junction channel activity involved in SA node cell-atrial cardiac muscle cell electrical coupling GO:0086020 NAS
    gap junction channel activity involved in cardiac conduction electrical coupling GO:0086075 IDA
    gap junction channel activity involved in cardiac conduction electrical coupling GO:0086075 IMP
    gap junction channel activity involved in atrial cardiac muscle cell-AV node cell electrical coupling GO:0086076 IBA
    gap junction channel activity involved in atrial cardiac muscle cell-AV node cell electrical coupling GO:0086076 IMP
    gap junction channel activity involved in atrial cardiac muscle cell-AV node cell electrical coupling GO:0086076 NAS
    gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling GO:0086077 IDA
    gap junction channel activity involved in bundle of His cell-Purkinje myocyte electrical coupling GO:0086078 IMP
    gap junction channel activity involved in bundle of His cell-Purkinje myocyte electrical coupling GO:0086078 ISS
    gap junction channel activity involved in Purkinje myocyte-ventricular cardiac muscle cell electrical coupling GO:0086079 NAS
    disordered domain specific binding GO:0097718 IEA
Biological Process
    angiogenesis GO:0001525 IEP
    negative regulation of glomerular filtration GO:0003105 IEA
    outflow tract morphogenesis GO:0003151 IMP
    endothelium development GO:0003158 IEA
    cardiac conduction system development GO:0003161 ISS
    mitral valve development GO:0003174 IMP
    pulmonary valve formation GO:0003193 IMP
    ventricular septum development GO:0003281 IMP
    atrial septum development GO:0003283 IMP
    potassium ion transport GO:0006813 IEA
    cell-cell signaling GO:0007267 IBA
    heart development GO:0007507 IBA
    cell communication by chemical coupling GO:0010643 IEA
    regulation of cell communication by electrical coupling GO:0010649 IEA
    positive regulation of cell communication by chemical coupling GO:0010652 IEA
    gap junction assembly GO:0016264 IDA
    gap junction assembly GO:0016264 IMP
    vasodilation GO:0042311 IEA
    negative regulation of blood pressure GO:0045776 IEA
    positive regulation of vasoconstriction GO:0045907 IEA
    artery morphogenesis GO:0048844 ISS
    transmembrane transport GO:0055085 IEA
    regulation of cardiac muscle contraction GO:0055117 IMP
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 ISS
    regulation of atrial cardiac muscle cell membrane depolarization GO:0060371 IMP
    regulation of ventricular cardiac muscle cell membrane depolarization GO:0060373 ISS
    SA node cell to atrial cardiac muscle cell communication by electrical coupling GO:0086021 NAS
    atrial cardiac muscle cell to AV node cell communication by electrical coupling GO:0086044 IBA
    atrial cardiac muscle cell to AV node cell communication by electrical coupling GO:0086044 IMP
    atrial cardiac muscle cell to AV node cell communication by electrical coupling GO:0086044 NAS
    AV node cell to bundle of His cell communication by electrical coupling GO:0086053 IMP
    bundle of His cell to Purkinje myocyte communication by electrical coupling GO:0086054 IMP
    bundle of His cell to Purkinje myocyte communication by electrical coupling GO:0086054 ISS
    Purkinje myocyte to ventricular cardiac muscle cell communication by electrical coupling GO:0086055 NAS
    cell communication by electrical coupling involved in cardiac conduction GO:0086064 IDA
    regulation of AV node cell action potential GO:0098904 IMP
    regulation of bundle of His cell action potential GO:0098905 IMP
    regulation of Purkinje myocyte action potential GO:0098906 IMP
    regulation of atrial cardiac muscle cell action potential GO:0098910 IMP
    vasomotion GO:1990029 IEA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    gap junction GO:0005921 IDA
    connexin complex GO:0005922 IBA
    connexin complex GO:0005922 IDA
    connexin complex GO:0005922 ISS
    intercalated disc GO:0014704 IDA
    intercalated disc GO:0014704 TAS
    cell projection GO:0042995 IEA
 Experiment description of studies that identified GJA5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GJA5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MARK1 4139
Two-hybrid Homo sapiens
2 ABHD16A 7920
Two-hybrid Homo sapiens
3 GJB2  
Two-hybrid Homo sapiens
4 SLC30A2  
Two-hybrid Homo sapiens
5 TMBIM6 7009
Two-hybrid Homo sapiens
6 LEPROTL1 23484
Two-hybrid Homo sapiens
7 YIPF6 286451
Two-hybrid Homo sapiens
8 GJA1 2697
Co-purification Homo sapiens
9 SLC35F1  
Two-hybrid Homo sapiens
10 FCER1G 2207
Two-hybrid Homo sapiens
11 MFSD5  
Two-hybrid Homo sapiens
12 IGFBP5 3488
Two-hybrid Homo sapiens
13 AAR2 25980
Two-hybrid Homo sapiens
14 EMP3 2014
Two-hybrid Homo sapiens
15 LEPROT 54741
Two-hybrid Homo sapiens
16 TMEM107 84314
Two-hybrid Homo sapiens
17 MAP1LC3B 81631
Affinity Capture-Western Homo sapiens
18 LHFPL2 10184
Two-hybrid Homo sapiens
19 PSMD2 5708
Affinity Capture-Western Homo sapiens
20 RPRM  
Two-hybrid Homo sapiens
21 SLC41A2 84102
Two-hybrid Homo sapiens
22 SLC13A4  
Two-hybrid Homo sapiens
23 BLCAP  
Two-hybrid Homo sapiens
24 SLC41A1 254428
Two-hybrid Homo sapiens
25 C4orf3  
Two-hybrid Homo sapiens
26 UBQLN4 56893
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
27 PGAP2  
Two-hybrid Homo sapiens
28 RIMS3  
Two-hybrid Homo sapiens
29 PRAF2 11230
Two-hybrid Homo sapiens
30 NKG7  
Two-hybrid Homo sapiens
31 TREX1  
Two-hybrid Homo sapiens
32 MS4A13  
Two-hybrid Homo sapiens
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