Gene description for Ap2s1
Gene name adaptor-related protein complex 2, sigma 1 subunit
Gene symbol Ap2s1
Other names/aliases AI043088
Species Mus musculus
 Database cross references - Ap2s1
ExoCarta ExoCarta_232910
Vesiclepedia VP_232910
Entrez Gene 232910
UniProt P62743  
 Ap2s1 identified in sEVs derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Ap2s1
Molecular Function
    protein binding GO:0005515 IPI
    clathrin adaptor activity GO:0035615 IEA
Biological Process
    intracellular protein transport GO:0006886 IEA
    vesicle-mediated transport GO:0016192 IBA
    vesicle-mediated transport GO:0016192 NAS
    synaptic vesicle endocytosis GO:0048488 IEA
    synaptic vesicle endocytosis GO:0048488 ISO
    clathrin-dependent endocytosis GO:0072583 NAS
    postsynaptic neurotransmitter receptor internalization GO:0098884 ISO
    postsynaptic neurotransmitter receptor internalization GO:0098884 NAS
Subcellular Localization
    cytoplasmic side of plasma membrane GO:0009898 NAS
    AP-2 adaptor complex GO:0030122 IDA
    AP-2 adaptor complex GO:0030122 ISO
    AP-2 adaptor complex GO:0030122 NAS
    intracellular membrane-bounded organelle GO:0043231 IBA
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 IEA
    postsynapse GO:0098794 IEA
    extrinsic component of presynaptic endocytic zone membrane GO:0098894 ISO
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Ap2s1 in sEVs
1
Experiment ID 264
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ap2s1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-MS Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Eps15 13858
Co-fractionation Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ap2s1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Developmental Biology IEA Reactome
Glutamate binding, activation of AMPA receptors and synaptic plasticity IEA Reactome
Immune System IEA Reactome
L1CAM interactions IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
MHC class II antigen presentation IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
PCP/CE pathway IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Recycling pathway of L1 IEA Reactome
Retrograde neurotrophin signalling IEA Reactome
Signal Transduction IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by WNT IEA Reactome
Trafficking of AMPA receptors IEA Reactome
Trafficking of GluR2-containing AMPA receptors IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
VLDLR internalisation and degradation IEA Reactome
WNT5A-dependent internalization of FZD2, FZD5 and ROR2 IEA Reactome
WNT5A-dependent internalization of FZD4 IEA Reactome





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