Gene description for Wipf1
Gene name WAS/WASL interacting protein family, member 1
Gene symbol Wipf1
Other names/aliases AI115543
D2Ertd120e
WIP
Waspip
Species Mus musculus
 Database cross references - Wipf1
ExoCarta ExoCarta_215280
Vesiclepedia VP_215280
Entrez Gene 215280
UniProt Q8K1I7  
 Wipf1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Wipf1
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    SH3 domain binding GO:0017124 ISO
    protein folding chaperone GO:0044183 ISO
Biological Process
    actin cytoskeleton organization GO:0030036 ISO
    actin filament-based movement GO:0030048 IBA
    actin filament-based movement GO:0030048 IDA
    positive regulation of protein export from nucleus GO:0046827 ISO
    response to other organism GO:0051707 IMP
Subcellular Localization
    ruffle GO:0001726 IEA
    actin filament GO:0005884 IBA
    actin filament GO:0005884 IDA
    actin filament GO:0005884 ISO
    actin cytoskeleton GO:0015629 IDA
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified Wipf1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Wipf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 Lrrk2  
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here