Gene description for ERCC2
Gene name excision repair cross-complementation group 2
Gene symbol ERCC2
Other names/aliases COFS2
EM9
TFIIH
TTD
XPD
Species Homo sapiens
 Database cross references - ERCC2
ExoCarta ExoCarta_2068
Vesiclepedia VP_2068
Entrez Gene 2068
HGNC 3434
MIM 126340
UniProt P18074  
 ERCC2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for ERCC2
Molecular Function
    DNA helicase activity GO:0003678 IBA
    damaged DNA binding GO:0003684 IBA
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    four-way junction helicase activity GO:0009378 IEA
    isomerase activity GO:0016853 IEA
    ATP hydrolysis activity GO:0016887 IEA
    protein-macromolecule adaptor activity GO:0030674 IPI
    double-stranded DNA helicase activity GO:0036121 IEA
    5'-3' DNA helicase activity GO:0043139 IDA
    5'-3' DNA helicase activity GO:0043139 TAS
    metal ion binding GO:0046872 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    forked DNA-dependent helicase activity GO:0061749 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
    single-stranded 3'-5' DNA helicase activity GO:1990518 IEA
Biological Process
    maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000462 IEA
    response to hypoxia GO:0001666 IEA
    in utero embryonic development GO:0001701 IEA
    transcription-coupled nucleotide-excision repair GO:0006283 IDA
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    nucleotide-excision repair GO:0006289 IMP
    nucleotide-excision repair GO:0006289 NAS
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IDA
    transcription elongation by RNA polymerase I GO:0006362 IEA
    transcription by RNA polymerase II GO:0006366 IBA
    transcription by RNA polymerase II GO:0006366 IDA
    transcription by RNA polymerase II GO:0006366 TAS
    transcription initiation at RNA polymerase II promoter GO:0006367 IDA
    apoptotic process GO:0006915 IMP
    response to oxidative stress GO:0006979 IMP
    chromosome segregation GO:0007059 IMP
    determination of adult lifespan GO:0008340 IEA
    UV protection GO:0009650 IEA
    post-embryonic development GO:0009791 IEA
    spinal cord development GO:0021510 IEA
    extracellular matrix organization GO:0030198 IEA
    bone mineralization GO:0030282 IEA
    central nervous system myelin formation GO:0032289 IEA
    DNA duplex unwinding GO:0032508 IEA
    multicellular organism growth GO:0035264 IEA
    hair cell differentiation GO:0035315 IMP
    embryonic cleavage GO:0040016 IEA
    erythrocyte maturation GO:0043249 IEA
    positive regulation of mitotic recombination GO:0045951 IBA
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    embryonic organ development GO:0048568 IEA
    hair follicle maturation GO:0048820 IEA
    hematopoietic stem cell differentiation GO:0060218 IEA
    hematopoietic stem cell proliferation GO:0071425 IEA
    intrinsic apoptotic signaling pathway by p53 class mediator GO:0072332 IEA
    chromatin looping GO:0140588 IEA
    regulation of mitotic cell cycle phase transition GO:1901990 IMP
Subcellular Localization
    transcription factor TFIIH core complex GO:0000439 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor TFIID complex GO:0005669 IDA
    transcription factor TFIIH holo complex GO:0005675 IDA
    transcription factor TFIIH holo complex GO:0005675 TAS
    cytoplasm GO:0005737 IDA
    spindle GO:0005819 IDA
    cytosol GO:0005829 IDA
    CAK-ERCC2 complex GO:0070516 IDA
    MMXD complex GO:0071817 IDA
 Experiment description of studies that identified ERCC2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ERCC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 GTF2F1 2962
Affinity Capture-Western Homo sapiens
4 CTPS1 1503
Co-fractionation Homo sapiens
5 ISG15 9636
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 ACAT1 38
Co-fractionation Homo sapiens
8 CCNH  
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 BRDT  
Affinity Capture-MS Homo sapiens
10 CDK7 1022
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CUL3 8452
Affinity Capture-MS Homo sapiens
12 TOPBP1 11073
Co-fractionation Homo sapiens
13 TP53 7157
Reconstituted Complex Homo sapiens
14 CIAO1 9391
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 WDR61 80349
Co-fractionation Homo sapiens
17 SSR2  
Affinity Capture-MS Homo sapiens
18 MFSD4  
Affinity Capture-MS Homo sapiens
19 UVSSA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 RPA2 6118
Proximity Label-MS Homo sapiens
21 DNA2  
Affinity Capture-MS Homo sapiens
22 TRIM66  
Affinity Capture-MS Homo sapiens
23 LMNB2 84823
Affinity Capture-MS Homo sapiens
24 RAD52  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 ATF7IP 55729
Affinity Capture-Western Homo sapiens
26 SPTA1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 PIDD1  
Affinity Capture-MS Homo sapiens
28 BRD2  
Affinity Capture-MS Homo sapiens
29 EGFR 1956
Affinity Capture-MS Homo sapiens
30 CLUH 23277
Co-fractionation Homo sapiens
31 GTF2H5  
Affinity Capture-MS Homo sapiens
32 PARK2  
Affinity Capture-MS Homo sapiens
33 EIF2A 83939
Co-fractionation Homo sapiens
34 GTF2H2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
35 POLR2A 5430
Affinity Capture-Western Homo sapiens
36 TRIM25 7706
Reconstituted Complex Homo sapiens
37 ERCC5  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
38 ERCC3  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
39 ERCC6  
Affinity Capture-Western Homo sapiens
40 MMS19 64210
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
41 GTF2H3  
Reconstituted Complex Homo sapiens
42 MNAT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
43 RPA3 6119
Proximity Label-MS Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 FAM96B 51647
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
46 GTF2H2C_2  
Two-hybrid Homo sapiens
47 KRAS 3845
Synthetic Lethality Homo sapiens
Negative Genetic Homo sapiens
48 C9orf72  
Affinity Capture-MS Homo sapiens
49 AR 367
Reconstituted Complex Homo sapiens
50 GTF2H1 2965
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
51 CDC7  
Affinity Capture-MS Homo sapiens
52 CETN2 1069
Affinity Capture-MS Homo sapiens
53 BRD3 8019
Affinity Capture-MS Homo sapiens
54 HERC5 51191
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which ERCC2 is involved
PathwayEvidenceSource
Cytosolic iron-sulfur cluster assembly IEA Reactome
Disease IEA Reactome
DNA Repair TAS Reactome
Dual Incision in GG-NER TAS Reactome
Dual incision in TC-NER TAS Reactome
Epigenetic regulation of gene expression IEA Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of Incision Complex in GG-NER TAS Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of TC-NER Pre-Incision Complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Gap-filling DNA repair synthesis and ligation in TC-NER TAS Reactome
Gene expression (Transcription) TAS Reactome
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway TAS Reactome
Global Genome Nucleotide Excision Repair (GG-NER) TAS Reactome
HIV Infection IEA Reactome
HIV Life Cycle IEA Reactome
HIV Transcription Elongation IEA Reactome
HIV Transcription Initiation IEA Reactome
Infectious disease IEA Reactome
Late Phase of HIV Life Cycle IEA Reactome
Metabolism IEA Reactome
Metabolism of RNA TAS Reactome
mRNA Capping TAS Reactome
Negative epigenetic regulation of rRNA expression IEA Reactome
NoRC negatively regulates rRNA expression IEA Reactome
Nucleotide Excision Repair TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE during HIV infection IEA Reactome
RNA Polymerase I Promoter Clearance TAS Reactome
RNA Polymerase I Promoter Escape TAS Reactome
RNA Polymerase I Transcription TAS Reactome
RNA Polymerase I Transcription Initiation TAS Reactome
RNA Polymerase I Transcription Termination TAS Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Tat-mediated elongation of the HIV-1 transcript IEA Reactome
TP53 Regulates Transcription of DNA Repair Genes TAS Reactome
Transcription of the HIV genome IEA Reactome
Transcription-Coupled Nucleotide Excision Repair (TC-NER) TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Viral Infection Pathways IEA Reactome





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