Gene description for Smpd1
Gene name sphingomyelin phosphodiesterase 1, acid lysosomal
Gene symbol Smpd1
Other names/aliases A-SMase
ASM
Zn-SMase
aSMase
Species Mus musculus
 Database cross references - Smpd1
ExoCarta ExoCarta_20597
Vesiclepedia VP_20597
Entrez Gene 20597
UniProt Q04519  
 Smpd1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Smpd1
Molecular Function
    sphingomyelin phosphodiesterase activity GO:0004767 ISO
    zinc ion binding GO:0008270 IDA
    zinc ion binding GO:0008270 ISO
    hydrolase activity, acting on glycosyl bonds GO:0016798 IEA
    phosphatidylcholine phospholipase C activity GO:0034480 IEA
    acid sphingomyelin phosphodiesterase activity GO:0061750 IBA
    acid sphingomyelin phosphodiesterase activity GO:0061750 IDA
    acid sphingomyelin phosphodiesterase activity GO:0061750 ISO
Biological Process
    plasma membrane repair GO:0001778 IDA
    plasma membrane repair GO:0001778 ISO
    plasma membrane repair GO:0001778 ISS
    ceramide metabolic process GO:0006672 IMP
    sphingomyelin catabolic process GO:0006685 IBA
    sphingomyelin catabolic process GO:0006685 IMP
    sphingomyelin catabolic process GO:0006685 ISO
    sphingomyelin catabolic process GO:0006685 ISS
    cholesterol metabolic process GO:0008203 IMP
    response to xenobiotic stimulus GO:0009410 IEA
    response to virus GO:0009615 ISO
    response to virus GO:0009615 ISS
    response to ionizing radiation GO:0010212 IMP
    response to ionizing radiation GO:0010212 ISO
    termination of signal transduction GO:0023021 IEA
    termination of signal transduction GO:0023021 ISO
    response to type I interferon GO:0034340 ISO
    response to type I interferon GO:0034340 ISS
    response to tumor necrosis factor GO:0034612 ISO
    response to tumor necrosis factor GO:0034612 ISS
    cellular response to UV GO:0034644 ISO
    cellular response to UV GO:0034644 ISS
    wound healing GO:0042060 ISO
    wound healing GO:0042060 ISS
    response to cocaine GO:0042220 IEA
    response to cocaine GO:0042220 ISO
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of MAP kinase activity GO:0043407 ISO
    negative regulation of MAPK cascade GO:0043409 IEA
    positive regulation of endocytosis GO:0045807 ISO
    positive regulation of endocytosis GO:0045807 ISS
    ceramide biosynthetic process GO:0046513 IBA
    ceramide biosynthetic process GO:0046513 IDA
    ceramide biosynthetic process GO:0046513 IMP
    ceramide biosynthetic process GO:0046513 ISO
    ceramide biosynthetic process GO:0046513 ISS
    positive regulation of viral entry into host cell GO:0046598 IEA
    positive regulation of viral entry into host cell GO:0046598 ISO
    symbiont entry into host cell GO:0046718 ISO
    symbiont entry into host cell GO:0046718 ISO
    symbiont entry into host cell GO:0046718 ISO
    symbiont entry into host cell GO:0046718 ISS
    response to interleukin-1 GO:0070555 ISO
    response to interleukin-1 GO:0070555 ISS
    cellular response to calcium ion GO:0071277 ISO
    cellular response to calcium ion GO:0071277 ISS
Subcellular Localization
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISO
    lysosome GO:0005764 IBA
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISO
    lysosome GO:0005764 ISS
    endosome GO:0005768 ISO
    lipid droplet GO:0005811 IEA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    endolysosome GO:0036019 ISO
    endolysosome GO:0036019 ISS
    lamellar body GO:0042599 IEA
    lamellar body GO:0042599 ISO
 Experiment description of studies that identified Smpd1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Smpd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Anxa4 11746
Co-fractionation Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here