Gene description for Rasa3
Gene name RAS p21 protein activator 3
Gene symbol Rasa3
Other names/aliases AI326412
C86362
E130011G04
GAPIII
scat
Species Mus musculus
 Database cross references - Rasa3
ExoCarta ExoCarta_19414
Vesiclepedia VP_19414
Entrez Gene 19414
UniProt Q60790  
 Rasa3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rasa3
Molecular Function
    GTPase activator activity GO:0005096 IEA
    calcium channel regulator activity GO:0005246 IEA
    intracellularly gated calcium channel activity GO:0015278 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    platelet activation GO:0030168 IEA
    cellular response to heat GO:0034605 ISO
    intracellular signal transduction GO:0035556 IEA
    negative regulation of Ras protein signal transduction GO:0046580 IEA
Subcellular Localization
    cytoplasmic side of plasma membrane GO:0009898 IEA
    cytoplasmic side of plasma membrane GO:0009898 ISO
 Experiment description of studies that identified Rasa3 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rasa3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sstr3  
Proximity Label-MS Mus musculus
View the network image/svg+xml



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