Gene description for Dennd5a
Gene name DENN/MADD domain containing 5A
Gene symbol Dennd5a
Other names/aliases 1500012B19Rik
AJ245569
AW496491
ORF37
Rab6ip1
mKIAA1091
Species Mus musculus
 Database cross references - Dennd5a
ExoCarta ExoCarta_19347
Vesiclepedia VP_19347
Entrez Gene 19347
UniProt Q6PAL8  
 Dennd5a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Dennd5a
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 ISO
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IBA
    small GTPase binding GO:0031267 IDA
Biological Process
    negative regulation of neuron projection development GO:0010977 ISO
    negative regulation of neuron projection development GO:0010977 ISS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IBA
    trans-Golgi network GO:0005802 IEA
    trans-Golgi network GO:0005802 ISO
    cytosol GO:0005829 IEA
    retromer complex GO:0030904 IEA
    retromer complex GO:0030904 ISO
 Experiment description of studies that identified Dennd5a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Dennd5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 St5  
Co-fractionation Mus musculus
2 Dennd5b  
Co-fractionation Mus musculus
3 Lmo2  
Two-hybrid Mus musculus
4 Madd  
Co-fractionation Mus musculus
5 Dennd3  
Co-fractionation Mus musculus
6 Dennd4c  
Co-fractionation Mus musculus
7 Dennd1c  
Co-fractionation Mus musculus
View the network image/svg+xml



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