Gene description for Ptbp1
Gene name polypyrimidine tract binding protein 1
Gene symbol Ptbp1
Other names/aliases AA407203
AL033359
HNRPI
PTB-1
PTB2
PTB3
PTB4
Ptb
pPTB
Species Mus musculus
 Database cross references - Ptbp1
ExoCarta ExoCarta_19205
Vesiclepedia VP_19205
Entrez Gene 19205
 Ptbp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ptbp1
Molecular Function
    single-stranded DNA endodeoxyribonuclease activity GO:0000014 ISO
    regulatory region RNA binding GO:0001069 ISO
    single-stranded DNA binding GO:0003697 ISO
    RNA binding GO:0003723 IPI
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 ISO
    protein binding GO:0005515 IPI
    pre-mRNA binding GO:0036002 ISO
    sequence-specific DNA binding GO:0043565 ISO
Biological Process
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISO
    regulation of alternative mRNA splicing, via spliceosome GO:0000381 ISS
    nuclear-transcribed mRNA catabolic process GO:0000956 ISO
    mRNA processing GO:0006397 IEA
    mRNA processing GO:0006397 ISO
    RNA splicing GO:0008380 IEA
    gene expression GO:0010467 IGI
    gene expression GO:0010467 IMP
    positive regulation of neuron projection development GO:0010976 ISO
    neurogenesis GO:0022008 IMP
    positive regulation of insulin secretion GO:0032024 ISO
    negative regulation of RNA splicing GO:0033119 ISO
    regulation of RNA splicing GO:0043484 IBA
    regulation of cell differentiation GO:0045595 IBA
    negative regulation of neuron differentiation GO:0045665 IDA
    positive regulation of translation GO:0045727 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IDA
    negative regulation of mRNA splicing, via spliceosome GO:0048025 IMP
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISO
    negative regulation of mRNA splicing, via spliceosome GO:0048025 ISS
    negative regulation of muscle cell differentiation GO:0051148 ISO
    negative regulation of muscle cell differentiation GO:0051148 ISS
    positive regulation of calcineurin-NFAT signaling cascade GO:0070886 ISO
    3'-UTR-mediated mRNA stabilization GO:0070935 ISO
    IRES-dependent viral translational initiation GO:0075522 ISO
    positive regulation of secretory granule organization GO:1904411 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IPI
    neuron projection terminus GO:0044306 ISO
 Experiment description of studies that identified Ptbp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ptbp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAVER2  
Affinity Capture-MS Homo sapiens
2 ZNFX1 57169
Affinity Capture-MS Homo sapiens
3 Fitm2  
Positive Genetic Mus musculus
4 VTI1B 10490
Affinity Capture-MS Homo sapiens
5 U2af2 22185
Co-fractionation Mus musculus
6 Kcna3  
Affinity Capture-MS Mus musculus
7 Kctd13  
Affinity Capture-MS Mus musculus
8 HNRNPM 4670
Affinity Capture-MS Homo sapiens
9 Tfe3  
Affinity Capture-MS Mus musculus
10 PTBP3 9991
Affinity Capture-MS Homo sapiens
11 SUGP2  
Affinity Capture-MS Homo sapiens
12 RAVER1 125950
Affinity Capture-MS Homo sapiens
13 Hnrnph1 59013
Co-fractionation Mus musculus
14 Tmem173  
Proximity Label-MS Mus musculus
15 HAUS5 23354
Affinity Capture-MS Homo sapiens
16 HEATR3 55027
Affinity Capture-MS Homo sapiens
17 MATR3 9782
Affinity Capture-MS Homo sapiens
18 Hnrnpf 98758
Co-fractionation Mus musculus
19 CIZ1  
Affinity Capture-MS Homo sapiens
20 Eed  
Affinity Capture-MS Mus musculus
21 IVNS1ABP  
Affinity Capture-MS Homo sapiens
22 Atg16l1  
Affinity Capture-MS Mus musculus
23 NSUN4  
Affinity Capture-MS Homo sapiens
24 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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