Gene description for Gnaq
Gene name guanine nucleotide binding protein, alpha q polypeptide
Gene symbol Gnaq
Other names/aliases 1110005L02Rik
6230401I02Rik
AA408290
AW060788
Dsk1
Dsk10
Galphaq
Gq
GqI
Species Mus musculus
 Database cross references - Gnaq
ExoCarta ExoCarta_14682
Vesiclepedia VP_14682
Entrez Gene 14682
UniProt P21279  
 Gnaq identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Mov neuroglial cells 15210972    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gnaq
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    G protein-coupled receptor binding GO:0001664 IEA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 TAS
    G protein activity GO:0003925 IDA
    G protein activity GO:0003925 IMP
    G protein activity GO:0003925 ISO
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 ISO
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
    enzyme regulator activity GO:0030234 IDA
    enzyme regulator activity GO:0030234 IMP
    enzyme regulator activity GO:0030234 ISO
    G-protein beta/gamma-subunit complex binding GO:0031683 IBA
    G-protein beta/gamma-subunit complex binding GO:0031683 IEA
    protein-containing complex binding GO:0044877 ISO
    metal ion binding GO:0046872 IEA
    alkylglycerophosphoethanolamine phosphodiesterase activity GO:0047391 ISO
Biological Process
    skeletal system development GO:0001501 IMP
    action potential GO:0001508 IMP
    negative regulation of protein kinase activity GO:0006469 ISO
    G protein-coupled receptor signaling pathway GO:0007186 IMP
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IDA
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IDA
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IGI
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 IMP
    phospholipase C-activating G protein-coupled receptor signaling pathway GO:0007200 ISO
    phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway GO:0007207 IGI
    glutamate receptor signaling pathway GO:0007215 IBA
    glutamate receptor signaling pathway GO:0007215 IMP
    heart development GO:0007507 IMP
    regulation of blood pressure GO:0008217 IGI
    post-embryonic development GO:0009791 IMP
    regulation of platelet activation GO:0010543 IMP
    neuron remodeling GO:0016322 IMP
    forebrain neuron development GO:0021884 IMP
    positive regulation of insulin secretion GO:0032024 IGI
    positive regulation of insulin secretion GO:0032024 IMP
    maternal behavior GO:0042711 IMP
    embryonic digit morphogenesis GO:0042733 IGI
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of potassium ion transport GO:0043267 ISO
    regulation of melanocyte differentiation GO:0045634 IMP
    developmental pigmentation GO:0048066 IMP
    positive regulation of smooth muscle cell proliferation GO:0048661 ISO
    protein stabilization GO:0050821 ISO
    phospholipase C-activating dopamine receptor signaling pathway GO:0060158 IGI
    regulation of canonical Wnt signaling pathway GO:0060828 ISO
    endothelin receptor signaling pathway GO:0086100 IGI
    ion channel modulating, G protein-coupled receptor signaling pathway GO:0099105 IGI
    cranial skeletal system development GO:1904888 IGI
    ligand-gated ion channel signaling pathway GO:1990806 IGI
    ligand-gated ion channel signaling pathway GO:1990806 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IEA
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 ISO
    heterotrimeric G-protein complex GO:0005834 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 ISO
    membrane GO:0016020 IDA
    dendrite GO:0030425 IDA
    nuclear membrane GO:0031965 IEA
    cell body GO:0044297 IDA
    postsynaptic cytosol GO:0099524 IDA
 Experiment description of studies that identified Gnaq in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors "Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ"
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gnaq
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rgs18  
Affinity Capture-Western Mus musculus
2 Tub  
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 PLCB3 5331
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
5 Mapt  
Affinity Capture-MS Mus musculus
6 Agap2  
Affinity Capture-MS Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Gnaq is involved
PathwayEvidenceSource
Acetylcholine regulates insulin secretion IEA Reactome
ADP signalling through P2Y purinoceptor 1 IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Chaperonin-mediated protein folding IEA Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding IEA Reactome
Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion IEA Reactome
Free fatty acids regulate insulin secretion IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (q) signalling events IEA Reactome
G-protein activation IEA Reactome
G-protein mediated events IEA Reactome
GPCR downstream signalling IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Integration of energy metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of proteins IEA Reactome
Opioid Signalling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
PLC beta mediated events IEA Reactome
Protein folding IEA Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Signal amplification IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Thromboxane signalling through TP receptor IEA Reactome
Turbulent (oscillatory, disturbed) flow shear stress activates signaling by PIEZO1 and integrins in endothelial cells IEA Reactome





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