Gene description for Eno3
Gene name enolase 3, beta muscle
Gene symbol Eno3
Other names/aliases Eno-3
MSE
Species Mus musculus
 Database cross references - Eno3
ExoCarta ExoCarta_13808
Vesiclepedia VP_13808
Entrez Gene 13808
UniProt P21550  
 Eno3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Eno3
Molecular Function
    magnesium ion binding GO:0000287 IEA
    phosphopyruvate hydratase activity GO:0004634 IBA
    phosphopyruvate hydratase activity GO:0004634 IDA
    phosphopyruvate hydratase activity GO:0004634 ISO
    phosphopyruvate hydratase activity GO:0004634 ISS
    identical protein binding GO:0042802 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 ISS
    canonical glycolysis GO:0061621 IGI
Subcellular Localization
    phosphopyruvate hydratase complex GO:0000015 IBA
    phosphopyruvate hydratase complex GO:0000015 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    membrane GO:0016020 ISO
 Experiment description of studies that identified Eno3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Eno3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fzr1  
Affinity Capture-MS Mus musculus
2 Atxn1  
Proximity Label-MS Mus musculus
3 Snapin  
Co-fractionation Mus musculus
4 Myh6  
Cross-Linking-MS (XL-MS) Mus musculus
5 Htt  
Affinity Capture-MS Mus musculus
6 HSD17B10 3028
Affinity Capture-MS Homo sapiens
7 Eno1 13806
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
8 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Eno3 is involved
PathwayEvidenceSource
Gluconeogenesis IEA Reactome
Glucose metabolism IEA Reactome
Glycolysis IEA Reactome
Metabolism IEA Reactome
Metabolism of carbohydrates IEA Reactome





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