Gene description for Ctbp1
Gene name C-terminal binding protein 1
Gene symbol Ctbp1
Other names/aliases BARS
CtBP1-L
CtBP1-S
CtBP3/BARS
D4S115h
D5H4S115
D5H4S115E
Species Mus musculus
 Database cross references - Ctbp1
ExoCarta ExoCarta_13016
Vesiclepedia VP_13016
Entrez Gene 13016
UniProt O88712  
 Ctbp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ctbp1
Molecular Function
    transcription coregulator binding GO:0001221 IBA
    transcription corepressor binding GO:0001222 IEA
    transcription corepressor binding GO:0001222 ISO
    chromatin binding GO:0003682 IDA
    transcription coactivator activity GO:0003713 IBA
    transcription corepressor activity GO:0003714 IBA
    transcription corepressor activity GO:0003714 IEA
    transcription corepressor activity GO:0003714 ISO
    protein binding GO:0005515 IPI
    oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor GO:0016616 IEA
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    PDZ domain binding GO:0030165 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    NAD binding GO:0051287 ISO
    NAD binding GO:0051287 ISS
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    lncRNA binding GO:0106222 IDA
    DNA-binding transcription factor binding GO:0140297 IBA
    DNA-binding transcription factor binding GO:0140297 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    Notch signaling pathway GO:0007219 IMP
    negative regulation of DNA-templated transcription GO:0045892 IDA
    synaptic vesicle endocytosis GO:0048488 IDA
    synaptic vesicle endocytosis GO:0048488 IMP
    white fat cell differentiation GO:0050872 IDA
    regulation of cell cycle GO:0051726 IEA
    regulation of cell cycle GO:0051726 ISO
    synaptic vesicle clustering GO:0097091 IDA
    synaptic vesicle clustering GO:0097091 IMP
    presynapse to nucleus signaling pathway GO:0099526 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    transcription repressor complex GO:0017053 IDA
    transcription repressor complex GO:0017053 ISO
    presynapse GO:0098793 ISO
    presynaptic active zone cytoplasmic component GO:0098831 IDA
    extrinsic component of presynaptic endocytic zone membrane GO:0098894 ISO
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
    glutamatergic synapse GO:0098978 ISO
    GABA-ergic synapse GO:0098982 IDA
 Experiment description of studies that identified Ctbp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ctbp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tnik  
Affinity Capture-MS Mus musculus
2 HDAC4  
Two-hybrid Homo sapiens
3 Pde4dip  
Affinity Capture-MS Mus musculus
4 Ikzf1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
5 Hdac2  
Two-hybrid Mus musculus
6 Kdm2b  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
7 CTBP2 1488
Affinity Capture-Western Homo sapiens
8 Atxn1  
Affinity Capture-MS Mus musculus
9 Kctd13  
Affinity Capture-MS Mus musculus
10 IKZF4  
Reconstituted Complex Homo sapiens
11 Glis2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Reconstituted Complex Mus musculus
12 Kcnma1  
Affinity Capture-MS Mus musculus
13 HIC1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
14 Zfpm1  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
15 BMPR2 659
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
16 Pou5f1  
Affinity Capture-MS Mus musculus
17 Zbtb7b  
Affinity Capture-MS Mus musculus
18 Mapt  
Affinity Capture-MS Mus musculus
19 Tia1  
Affinity Capture-MS Mus musculus
20 Zeb1  
Two-hybrid Mus musculus
21 Fancd2  
Affinity Capture-MS Mus musculus
22 Hdac9  
Two-hybrid Mus musculus
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