Gene description for COL2A1
Gene name collagen, type II, alpha 1
Gene symbol COL2A1
Other names/aliases ANFH
AOM
COL11A3
SEDC
STL1
Species Homo sapiens
 Database cross references - COL2A1
ExoCarta ExoCarta_1280
Vesiclepedia VP_1280
Entrez Gene 1280
HGNC 2200
MIM 120140
UniProt P02458  
 COL2A1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for COL2A1
Molecular Function
    protein binding GO:0005515 IPI
    extracellular matrix structural constituent conferring tensile strength GO:0030020 HDA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IBA
    extracellular matrix structural constituent conferring tensile strength GO:0030020 IC
    extracellular matrix structural constituent conferring tensile strength GO:0030020 RCA
    MHC class II protein binding GO:0042289 IPI
    protein homodimerization activity GO:0042803 IPI
    proteoglycan binding GO:0043394 IDA
    metal ion binding GO:0046872 IEA
    platelet-derived growth factor binding GO:0048407 IDA
Biological Process
    skeletal system development GO:0001501 IMP
    cartilage condensation GO:0001502 IEA
    tissue homeostasis GO:0001894 IEA
    endochondral ossification GO:0001958 IEA
    chondrocyte differentiation GO:0002062 IEA
    heart morphogenesis GO:0003007 IEA
    proteoglycan metabolic process GO:0006029 IEA
    central nervous system development GO:0007417 IEA
    visual perception GO:0007601 IMP
    sensory perception of sound GO:0007605 IMP
    regulation of gene expression GO:0010468 IEA
    extracellular matrix organization GO:0030198 IBA
    collagen fibril organization GO:0030199 IMP
    inner ear morphogenesis GO:0042472 IEA
    cartilage development GO:0051216 TAS
    roof of mouth development GO:0060021 IEA
    limb bud formation GO:0060174 IEA
    embryonic skeletal joint morphogenesis GO:0060272 IMP
    cartilage development involved in endochondral bone morphogenesis GO:0060351 IEA
    otic vesicle development GO:0071599 IEA
    cellular response to BMP stimulus GO:0071773 IEA
    anterior head development GO:0097065 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IEA
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 IEA
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 TAS
    collagen type II trimer GO:0005585 IBA
    collagen type II trimer GO:0005585 IDA
    collagen type XI trimer GO:0005592 NAS
    basement membrane GO:0005604 IEA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IMP
    endoplasmic reticulum lumen GO:0005788 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IBA
    collagen-containing extracellular matrix GO:0062023 NAS
 Experiment description of studies that identified COL2A1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for COL2A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDGFA 5154
Reconstituted Complex Homo sapiens
2 MMP2 4313
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RELA 5970
Two-hybrid Homo sapiens
4 C1QTNF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 COL9A1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 BMP2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 ITGA2B 3674
Reconstituted Complex Homo sapiens
8 C1QTNF9B  
Affinity Capture-MS Homo sapiens
9 COLGALT2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PDIA2 64714
Reconstituted Complex Homo sapiens
11 HSPA8 3312
Cross-Linking-MS (XL-MS) Homo sapiens
12 MMP9 4318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 NXPH2  
Affinity Capture-MS Homo sapiens
14 C1QTNF9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CNPY2 10330
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 P4HA1 5033
Affinity Capture-MS Homo sapiens
17 Igfbp7  
Affinity Capture-Western Mus musculus
18 FKBP9 11328
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 PLOD1 5351
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 PKD1 5310
Reconstituted Complex Homo sapiens
21 MAG  
Reconstituted Complex Homo sapiens
22 HYAL1 3373
Two-hybrid Homo sapiens
23 PDGFB  
Reconstituted Complex Homo sapiens
Far Western Homo sapiens
24 LAIR2  
Affinity Capture-MS Homo sapiens
25 TGFB1 7040
Reconstituted Complex Homo sapiens
26 C1QTNF1 114897
Affinity Capture-MS Homo sapiens
27 CHODL 140578
Affinity Capture-MS Homo sapiens
28 ANXA5 308
Reconstituted Complex Homo sapiens
29 COL8A2 1296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 THBS1 7057
Reconstituted Complex Homo sapiens
31 COL8A1 1295
Affinity Capture-MS Homo sapiens
32 RPL36AL 6166
Affinity Capture-MS Homo sapiens
33 PRELP 5549
Reconstituted Complex Homo sapiens
34 C1QC 714
Affinity Capture-MS Homo sapiens
35 OSCAR  
Affinity Capture-MS Homo sapiens
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