Gene description for MGAT4A
Gene name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
Gene symbol MGAT4A
Other names/aliases GNT-IV
GNT-IVA
GnT-4a
Species Homo sapiens
 Database cross references - MGAT4A
ExoCarta ExoCarta_11320
Vesiclepedia VP_11320
Entrez Gene 11320
HGNC 7047
MIM 604623
UniProt Q9UM21  
 MGAT4A identified in sEVs derived from the following tissue/cell type
Saliva 19199708    
 Gene ontology annotations for MGAT4A
Molecular Function
    acetylglucosaminyltransferase activity GO:0008375 IBA
    alanine-glyoxylate transaminase activity GO:0008453 ISS
    alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity GO:0008454 IDA
    alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity GO:0008454 TAS
    protein homodimerization activity GO:0042803 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    protein glycosylation GO:0006486 IDA
    protein N-linked glycosylation GO:0006487 IBA
    N-glycan processing GO:0006491 ISS
    viral protein processing GO:0019082 TAS
    glyoxylate metabolic process GO:0046487 ISS
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    peroxisome GO:0005777 ISS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum lumen GO:0005788 TAS
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 IBA
    Golgi stack GO:0005795 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MGAT4A in sEVs
1
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for MGAT4A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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