Gene description for Rap1a
Gene name RAS-related protein-1a
Gene symbol Rap1a
Other names/aliases AI848598
G-22K
Krev-1
Rap1
Species Mus musculus
 Database cross references - Rap1a
ExoCarta ExoCarta_109905
Vesiclepedia VP_109905
Entrez Gene 109905
UniProt P62835  
 Rap1a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rap1a
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    G protein activity GO:0003925 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 ISO
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    GDP binding GO:0019003 IBA
    small GTPase binding GO:0031267 IPI
    protein-containing complex binding GO:0044877 ISO
Biological Process
    small GTPase-mediated signal transduction GO:0007264 IDA
    nervous system development GO:0007399 IEA
    positive regulation of neuron projection development GO:0010976 IDA
    positive regulation of neuron projection development GO:0010976 ISO
    positive regulation of neuron projection development GO:0010976 ISS
    synaptic vesicle exocytosis GO:0016079 IGI
    Rap protein signal transduction GO:0032486 IBA
    Rap protein signal transduction GO:0032486 ISO
    Rap protein signal transduction GO:0032486 ISS
    negative regulation of collagen biosynthetic process GO:0032966 ISO
    nerve growth factor signaling pathway GO:0038180 ISO
    nerve growth factor signaling pathway GO:0038180 ISS
    positive regulation of GTPase activity GO:0043547 ISS
    positive regulation of protein kinase activity GO:0045860 ISS
    positive regulation of D-glucose import GO:0046326 ISO
    positive regulation of phagocytosis GO:0050766 ISO
    positive regulation of Fc receptor mediated stimulatory signaling pathway GO:0060369 ISO
    establishment of endothelial barrier GO:0061028 ISO
    establishment of endothelial barrier GO:0061028 ISS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IGI
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISS
    cellular response to cAMP GO:0071320 IBA
    cellular response to cAMP GO:0071320 ISO
    cellular response to cAMP GO:0071320 ISS
    cellular response to organic cyclic compound GO:0071407 ISO
    cellular response to xenobiotic stimulus GO:0071466 ISO
    protein localization to plasma membrane GO:0072659 ISS
    response to antineoplastic agent GO:0097327 ISO
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 IEA
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 ISO
    regulation of cell junction assembly GO:1901888 ISO
    regulation of cell junction assembly GO:1901888 ISS
    cellular response to nerve growth factor stimulus GO:1990090 ISO
    cellular response to nerve growth factor stimulus GO:1990090 ISS
    negative regulation of synaptic vesicle exocytosis GO:2000301 IBA
    negative regulation of synaptic vesicle exocytosis GO:2000301 IGI
    positive regulation of vasculogenesis GO:2001214 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    early endosome GO:0005769 ISO
    early endosome GO:0005769 ISS
    late endosome GO:0005770 ISO
    late endosome GO:0005770 ISS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    cell junction GO:0030054 IDA
    guanyl-nucleotide exchange factor complex GO:0032045 IDA
    neuron projection GO:0043005 IDA
    myelin sheath GO:0043209 HDA
    phagocytic vesicle GO:0045335 ISO
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    anchoring junction GO:0070161 IEA
    sperm midpiece GO:0097225 IDA
    presynapse GO:0098793 IEA
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rap1a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
7
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rap1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Sstr3  
Proximity Label-MS Mus musculus
3 Grin2b  
Affinity Capture-Western Mus musculus
4 Foxp3  
Affinity Capture-MS Mus musculus
5 Shank3  
Affinity Capture-MS Mus musculus
6 Unk  
Affinity Capture-RNA Mus musculus
7 Kcnma1  
Affinity Capture-MS Mus musculus
8 Grin1  
Affinity Capture-Western Mus musculus
9 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
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